Plasmid vector pBR322 and its special-purpose derivatives--a review - PubMed (original) (raw)
Plasmid vector pBR322 and its special-purpose derivatives--a review
P Balbás et al. Gene. 1986.
Abstract
The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA molecules in Escherichia coli. This 4363-bp DNA molecule has been extensively used as a cloning vehicle because of its simplicity and the availability of its nucleotide sequence. The widespread use of pBR322 has prompted numerous studies into its molecular structure and function. These studies revealed two features that detract from the plasmid's effectiveness as a cloning vector: plasmid instability in the absence of selection and, the lack of a direct selection scheme for recombinant DNA molecules. Several vectors based on pBR322 have been constructed to overcome these limitations and to extend the vector's versatility to accommodate special cloning purposes. The objective of this review is to provide a survey of these derivative vectors and to summarize information currently available on pBR322.
Similar articles
- Vectors with restriction-site banks. I. pJRD158, a 3903-bp plasmid containing 28 unique cloning sites.
Davison J, Heusterspreute M, Merchez M, Brunel F. Davison J, et al. Gene. 1984 Jun;28(3):311-8. doi: 10.1016/0378-1119(84)90148-3. Gene. 1984. PMID: 6086459 - The site-specific deletion in plasmid pBR322.
Garaev MM, Bobkov AF, Bobkova AF, Kalinin VN, Smirnov VD, Khudakov YuE, Tikchonenko TI. Garaev MM, et al. Gene. 1982 Apr;18(1):21-8. doi: 10.1016/0378-1119(82)90052-x. Gene. 1982. PMID: 6286416 - Back to basics: pBR322 and protein expression systems in E. coli.
Balbás P, Bolívar F. Balbás P, et al. Methods Mol Biol. 2004;267:77-90. doi: 10.1385/1-59259-774-2:077. Methods Mol Biol. 2004. PMID: 15269416 Review. - Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328.
Covarrubias L, Cervantes L, Covarrubias A, Soberón X, Vichido I, Blanco A, Kupersztoch-Portnoy YM, Bolivar F. Covarrubias L, et al. Gene. 1981 Jan-Feb;13(1):25-35. doi: 10.1016/0378-1119(81)90040-8. Gene. 1981. PMID: 6263753 - The plasmid, pBR322.
Balbas P, Soberon X, Bolivar F, Rodriguez RL. Balbas P, et al. Biotechnology. 1988;10:5-41. doi: 10.1016/b978-0-409-90042-2.50007-6. Biotechnology. 1988. PMID: 3061523 Review. No abstract available.
Cited by
- RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase.
Rådström P, Swedberg G. Rådström P, et al. Antimicrob Agents Chemother. 1988 Nov;32(11):1684-92. doi: 10.1128/AAC.32.11.1684. Antimicrob Agents Chemother. 1988. PMID: 3075438 Free PMC article. - Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription.
Bell A, Gaston K, Williams R, Chapman K, Kolb A, Buc H, Minchin S, Williams J, Busby S. Bell A, et al. Nucleic Acids Res. 1990 Dec 25;18(24):7243-50. doi: 10.1093/nar/18.24.7243. Nucleic Acids Res. 1990. PMID: 2259621 Free PMC article. - Inhibition of cell growth and stable DNA replication by overexpression of the bla gene of plasmid pBR322 in Escherichia coli.
Katayama T, Nagata T. Katayama T, et al. Mol Gen Genet. 1990 Sep;223(3):353-60. doi: 10.1007/BF00264440. Mol Gen Genet. 1990. PMID: 2270075 - Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate.
Zhong Z, Toukdarian A, Helinski D, Knauf V, Sykes S, Wilkinson JE, O'Bryne C, Shea T, DeLoughery C, Caspi R. Zhong Z, et al. Appl Environ Microbiol. 2001 Dec;67(12):5771-9. doi: 10.1128/AEM.67.12.5771-5779.2001. Appl Environ Microbiol. 2001. PMID: 11722934 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources