Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection - PubMed (original) (raw)
Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection
R Heumann et al. J Cell Biol. 1987 Jun.
Abstract
The intact sciatic nerve contains levels of nerve growth factor (NGF) that are comparable to those of densely innervated peripheral target tissues of NGF-responsive (sympathetic and sensory) neurons. There, the high NGF levels are reflected by correspondingly high mRNANGF levels. In the intact sciatic nerve, mRNANGF levels were very low, thus indicating that the contribution of locally synthesized NGF by nonneuronal cells is small. However, after transection an increase of up to 15-fold in mRNANGF was measured in 4-mm segments collected both proximally and distally to the transection site. Distally to the transection site, augmented mRNANGF levels occurred in all three 4-mm segments from 6 h to 2 wk after transection, the longest time period investigated. The augmented local NGF synthesis after transection was accompanied by a reexpression of NGF receptors by Schwann cells (NGF receptors normally disappear shortly after birth). Proximal to the transection site, the augmented NGF synthesis was restricted to the very end of the nerve stump that acts as a "substitute target organ" for the regenerating NGF-responsive nerve fibers. While the mRNANGF levels in the nerve stump correspond to those of a densely innervated peripheral organ, the volume is too small to fully replace the lacking supply from the periphery. This is reflected by the fact that in the more proximal part of the transected sciatic nerve, where mRNANGF remained unchanged, the NGF levels reached only 40% of control values. In situ hybridization experiments demonstrated that after transection all nonneuronal cells express mRNANGF and not only those ensheathing the nerve fibers of NGF-responsive neurons.
Similar articles
- Differential regulation of mRNA encoding nerve growth factor and its receptor in rat sciatic nerve during development, degeneration, and regeneration: role of macrophages.
Heumann R, Lindholm D, Bandtlow C, Meyer M, Radeke MJ, Misko TP, Shooter E, Thoenen H. Heumann R, et al. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8735-9. doi: 10.1073/pnas.84.23.8735. Proc Natl Acad Sci U S A. 1987. PMID: 2825206 Free PMC article. - Induction of nerve growth factor receptor in Schwann cells after axotomy.
Taniuchi M, Clark HB, Johnson EM Jr. Taniuchi M, et al. Proc Natl Acad Sci U S A. 1986 Jun;83(11):4094-8. doi: 10.1073/pnas.83.11.4094. Proc Natl Acad Sci U S A. 1986. PMID: 3012551 Free PMC article. - [Changes of nerve growth factor (NGF) content in injured peripheral nerve during regeneration: local synthesis of NGF by Schwann cells].
Ikegami R. Ikegami R. Nihon Seikeigeka Gakkai Zasshi. 1990 Jul;64(7):612-22. Nihon Seikeigeka Gakkai Zasshi. 1990. PMID: 1700037 Japanese. - Nerve growth factor: cellular localization and regulation of synthesis.
Thoenen H, Bandtlow C, Heumann R, Lindholm D, Meyer M, Rohrer H. Thoenen H, et al. Cell Mol Neurobiol. 1988 Mar;8(1):35-40. doi: 10.1007/BF00712909. Cell Mol Neurobiol. 1988. PMID: 3042143 Free PMC article. Review. - Regulation of the synthesis of nerve growth factor.
Heumann R. Heumann R. J Exp Biol. 1987 Sep;132:133-50. doi: 10.1242/jeb.132.1.133. J Exp Biol. 1987. PMID: 3323396 Review.
Cited by
- Compartmental neurodegeneration and synaptic plasticity in the Wld(s) mutant mouse.
Gillingwater TH, Ribchester RR. Gillingwater TH, et al. J Physiol. 2001 Aug 1;534(Pt 3):627-39. doi: 10.1111/j.1469-7793.2001.00627.x. J Physiol. 2001. PMID: 11483696 Free PMC article. Review. - Neuromuscular Junction Changes in a Mouse Model of Charcot-Marie-Tooth Disease Type 4C.
Cipriani S, Phan V, Médard JJ, Horvath R, Lochmüller H, Chrast R, Roos A, Spendiff S. Cipriani S, et al. Int J Mol Sci. 2018 Dec 17;19(12):4072. doi: 10.3390/ijms19124072. Int J Mol Sci. 2018. PMID: 30562927 Free PMC article. - Macrophage biology in the peripheral nervous system after injury.
Zigmond RE, Echevarria FD. Zigmond RE, et al. Prog Neurobiol. 2019 Feb;173:102-121. doi: 10.1016/j.pneurobio.2018.12.001. Epub 2018 Dec 21. Prog Neurobiol. 2019. PMID: 30579784 Free PMC article. Review. - Interleukin 12: a potential link between nerve cells and the immune response in inflammatory disorders.
Turka LA, Goodman RE, Rutkowski JL, Sima AA, Merry A, Mitra RS, Wrone-Smith T, Toews G, Strieter RM, Nickoloff BJ. Turka LA, et al. Mol Med. 1995 Sep;1(6):690-9. Mol Med. 1995. PMID: 8529135 Free PMC article. - Differences in glial, synaptic and motoneuron responses in the facial nucleus of the rat brainstem following facial nerve resection and nerve suture reanastomosis.
Guntinas-Lichius O, Neiss WF, Gunkel A, Stennert E. Guntinas-Lichius O, et al. Eur Arch Otorhinolaryngol. 1994;251(7):410-7. doi: 10.1007/BF00181967. Eur Arch Otorhinolaryngol. 1994. PMID: 7857629
References
- Acta Neuropathol. 1967 Jan 2;7(3):218-31 - PubMed
- Physiol Rev. 1968 Jul;48(3):534-69 - PubMed
- Histochem J. 1970 May;2(3):209-18 - PubMed
- J Mol Biol. 1975 Aug 25;96(4):745-66 - PubMed
- Brain Res. 1976 Jun 11;109(2):271-84 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources