Coordinate regulation of phospholipid biosynthesis by serine in Saccharomyces cerevisiae - PubMed (original) (raw)

Coordinate regulation of phospholipid biosynthesis by serine in Saccharomyces cerevisiae

M J Homann et al. J Bacteriol. 1987 Jul.

Abstract

The addition of L-serine to inositol-containing growth medium repressed membrane-associated CDPdiacylglycerol synthase (CTP:phosphatidate cytidylyltransferase, EC 2.7.7.41) and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase, EC 2.7.8.8) activities and subunit levels in wild-type Saccharomyces cerevisiae. Enzyme activities and subunit levels were not repressed when inositol was absent from the growth medium. The addition of L-serine to the growth medium did not affect the phospholipid composition of wild-type cells. CDPdiacylglycerol synthase and phosphatidylserine synthase were not regulated in the S. cerevisiae inositol biosynthesis ino2, ino4, and opi1 regulatory mutants, suggesting that regulation by inositol plus L-serine is coupled to inositol synthesis. Inositol and L-serine did not affect the activities of purified CDPdiacylglycerol synthase and phosphatidylserine synthase. The addition of compounds structurally related to L-serine to the growth medium of wild-type cells also resulted in a repression of CDPdiacylglycerol synthase and phosphatidylserine synthase but only in the presence of inositol. Phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was not regulated by inositol plus L-serine.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Appl Environ Microbiol. 1982 Jan;43(1):81-5 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Can J Microbiol. 1981 Nov;27(11):1140-9 - PubMed
    1. Eur J Biochem. 1982 Nov 15;128(2-3):589-95 - PubMed
    1. Mol Cell Biol. 1984 Nov;4(11):2479-85 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources