Calcium- and cyclic AMP-dependent chloride secretion in human colonic epithelia - PubMed (original) (raw)

Calcium- and cyclic AMP-dependent chloride secretion in human colonic epithelia

A W Cuthbert et al. Br J Pharmacol. 1987 Jul.

Abstract

Three stable epithelial cell lines (HCA-7, HCA-7-Col 1 and HCA-7-Col 3) all derived from the same human adenocarcinoma have been cultured on collagen-coated Millipore filters. These epithelial monolayers have been used to record short circuit current (SCC) in response to of secretagogues. Similar monolayers, but grown on plastic dishes, were used for measurements of tissue cyclic AMP. Lysylbradykinin, applied to either side of the monolayers, increased SCC in HCA-7 cells but had little effect on the other two lines. The responses showed rapid desensitization, which could be prevented by cooling to 4 degrees C. Responses to kinin were not significantly attenuated by piroxicam, an inhibitor of cyclo-oxygenase. Other secretagogues, vasoactive intestinal polypeptide (VIP) and carbachol also increased SCC in monolayers. The responses to VIP were greatest in HCA-7-Col 1 monolayers while responses were virtually absent in HCA-7-Col 3. A similar profile was seen with carbachol except that responses of HCA-7 and HCA-7-Col 1 monolayers were more equal. With one exception the responses to VIP and carbachol showed sidedness, acting only from the basolateral side. The effects of the secretagogues were inhibited by piretanide, a loop diuretic, applied basolaterally. It is presumed that SCC responses represent electrogenic chloride secretion. Treatment with forskolin increased SCC in HCA-7 and HCA-7-Col 1 monolayers with little effect in HCA-7-Col 3. Nevertheless cyclic AMP levels were elevated most in HCA-7-Col 3 and least in HCA-7-Col 1 monolayers, in reciprocal relationship to the functional response. A23187 increased SCC when applied to HCA-7 and HCA-7-Col 3 monolayers with little effect on HCA-7-Col 1. The differential responses of the three human cell lines provide unique opportunities to discover the functional responsibilities of entities involved in the chloride secretory process. HCA-7-Col 3 cells which generate high levels of cyclic AMP in response to forskolin but which fail to show a substantial chloride secretory response may be a useful model of some disease conditions.

PubMed Disclaimer

Similar articles

• Cyclic AMP and Ca2+ interactions affecting epithelial chloride secretion in human cultured colonic epithelia.
  MacVinish LJ, Pickles RJ, Cuthbert AW. MacVinish LJ, et al. Br J Pharmacol. 1993 Feb;108(2):462-8. doi: 10.1111/j.1476-5381.1993.tb12826.x. Br J Pharmacol. 1993. PMID: 8383565 Free PMC article.
• Inhibition of cyclic AMP-dependent chloride secretion by PP receptors and alpha 2-adrenoceptors in a human colonic epithelial cell line.
  Holliday ND, Tough IR, Cox HM. Holliday ND, et al. Naunyn Schmiedebergs Arch Pharmacol. 1997 Feb;355(2):183-9. doi: 10.1007/pl00004930. Naunyn Schmiedebergs Arch Pharmacol. 1997. PMID: 9050010
• Relation of anion secretory activity to intracellular Ca2+ in response to lysylbradykinin and histamine in a cultured human colonic epithelium.
  Pickles RJ, Cuthbert AW. Pickles RJ, et al. Eur J Pharmacol. 1991 Jun 18;199(1):77-91. doi: 10.1016/0014-2999(91)90639-8. Eur J Pharmacol. 1991. PMID: 1893929
• Calcitonin gene-related peptide receptors in human gastrointestinal epithelia.
  Cox HM, Tough IR. Cox HM, et al. Br J Pharmacol. 1994 Dec;113(4):1243-8. doi: 10.1111/j.1476-5381.1994.tb17131.x. Br J Pharmacol. 1994. PMID: 7889279 Free PMC article.
• The role of cyclic nucleotides and calcium in the regulation of chloride transport.
  Berridge MJ. Berridge MJ. Ann N Y Acad Sci. 1980;341:156-71. doi: 10.1111/j.1749-6632.1980.tb47170.x. Ann N Y Acad Sci. 1980. PMID: 6249146 Review. No abstract available.

Cited by

• The functional investigation of a human adenocarcinoma cell line, stably transfected with the neuropeptide Y Y1 receptor.
  Holliday ND, Cox HM. Holliday ND, et al. Br J Pharmacol. 1996 Sep;119(2):321-9. doi: 10.1111/j.1476-5381.1996.tb15989.x. Br J Pharmacol. 1996. PMID: 8886416 Free PMC article.
• Ion-transporting activity in the murine colonic epithelium of normal animals and animals with cystic fibrosis.
  Cuthbert AW, MacVinish LJ, Hickman ME, Ratcliff R, Colledge WH, Evans MJ. Cuthbert AW, et al. Pflugers Arch. 1994 Oct;428(5-6):508-15. doi: 10.1007/BF00374572. Pflugers Arch. 1994. PMID: 7838673
• Ion transport in cultured epithelia from human sweat glands: comparison of normal and cystic fibrosis tissues.
  Brayden DJ, Pickles RJ, Cuthbert AW. Brayden DJ, et al. Br J Pharmacol. 1991 Jan;102(1):57-64. doi: 10.1111/j.1476-5381.1991.tb12132.x. Br J Pharmacol. 1991. PMID: 1646063 Free PMC article.
• Altered sensitivity to amiloride in cystic fibrosis. Observations using cultured sweat glands.
  Cuthbert AW, Brayden DJ, Dunne A, Smyth RL, Wallwork J. Cuthbert AW, et al. Br J Clin Pharmacol. 1990 Feb;29(2):227-34. doi: 10.1111/j.1365-2125.1990.tb03624.x. Br J Clin Pharmacol. 1990. PMID: 2306415 Free PMC article.
• Chloride channels and anion fluxes in a human colonic epithelium (HCA-7).
  Henderson RM, Ashford ML, MacVinish LJ, Cuthbert AW. Henderson RM, et al. Br J Pharmacol. 1992 May;106(1):109-14. doi: 10.1111/j.1476-5381.1992.tb14301.x. Br J Pharmacol. 1992. PMID: 1380377 Free PMC article.

References

1. 1. Cancer Res. 1979 Mar;39(3):1020-5 - PubMed
2. 1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
3. 1. Cancer Res. 1981 May;41(5):1751-6 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3363-7 - PubMed
5. 1. Science. 1982 Dec 17;218(4578):1219-21 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Wiley

• Research Materials

  • NCI CPTC Antibody Characterization Program