Effects of Probiotic Bacillus as an Alternative of Antibiotics on Digestive Enzymes Activity and Intestinal Integrity of Piglets - PubMed (original) (raw)

Effects of Probiotic Bacillus as an Alternative of Antibiotics on Digestive Enzymes Activity and Intestinal Integrity of Piglets

Shenglan Hu et al. Front Microbiol. 2018.

Abstract

The previous study in our team found that supplementation of probiotic Bacillus amyloliquefaciens (Ba) instead of antibiotics promote growth performance of piglets. Hence, the present study was carried out to further demonstrate the effect of Ba replacement of antibiotics on digestive and absorption enzyme activity and intestinal microbiota population of piglets. A total of 90 piglets were selected and divided into three groups: G1 group was fed with basal diet supplemented with 150 mg/Kg aureomycin, G2 group was fed with 1 × 108 cfu/Kg Ba and half dose of aureomycin, G3 group was used the diet with 2 × 108cfu/Kg Ba replaced aureomycin. Each treatment had three replications of 10 pigs per pen. Results indicated that Ba replacement significantly increased the activities of amylase, disaccharides and Na+/K+-ATPase. And chymotrypsin activity in different section of intestine was dramatically enhanced in half replacement of aureomycin with Ba. Moreover, Ba replacement maintained the intestinal integrity with the significantly decreased activity of DAO compared with aureomycin group. Besides, supplementation with Ba increased the β-diversity of intestinal microbiota. Taken together, the current study indicated that diet supplementation with Ba instead of aureomycin increased the growth performance of piglets by improving the digestive and absorb enzyme activities, enhancing the intestinal integrity and regulating the population of intestinal micrbiota.

Keywords: Bacillus amyloliquefaciens; antibiotics; digestive enzyme activity; intestinal integrity; piglets.

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Figures

FIGURE 1

FIGURE 1

Effects of Ba on chymotrypsin activity in contents of duodenum (A), jejunum (B), and ileum (C). Results were expressed as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 2

FIGURE 2

Effects of Ba on amylase activity in contents of duodenum (A), jejunum (B), and ileum (C). Results were expressed as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 3

FIGURE 3

Effects of Ba on activity of lipase in contents of duodenum (A) and jejunum (B). Results were expressed as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 4

FIGURE 4

Effects of Ba on activity of trypsin in contents of duodenum (A) and jejunum (B). Results were expressed as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 5

FIGURE 5

Effects of Ba on the activity of sucrase (A), lactase (B), maltase (C), AKPase (D), Na +, K +-ATPase (E), γ-GT (F), and DAO (G) in jejunal mucosa of piglets. Results were expressed as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 6

FIGURE 6

(A) Effects of Ba on the expression of SGLT1 and PEPT1 in jejuna mucosa of piglets. (B) Results were given as mean ± SEM, n = 6. a and b means significantly different (p < 0.05).

FIGURE 7

FIGURE 7

Effects of Ba on α-diversity of microbial population in piglets. Shannon (A), Chao1 (B), PD whole tree (C), and observed species (D) was analyzed.

FIGURE 8

FIGURE 8

Effects of Ba on β-diversity of microbial population in piglets. Unweight unifrac (A) and weighted unifrac (B) was analyzed.

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