Bordetella bronchiseptica Bcr4 antagonizes the negative regulatory function of BspR via its role in type III secretion - PubMed (original) (raw)
. 2018 Dec;62(12):743-754.
doi: 10.1111/1348-0421.12659. Epub 2018 Dec 14.
Affiliations
- PMID: 30407657
- DOI: 10.1111/1348-0421.12659
Free article
Bordetella bronchiseptica Bcr4 antagonizes the negative regulatory function of BspR via its role in type III secretion
Ryutaro Nishimura et al. Microbiol Immunol. 2018 Dec.
Free article
Abstract
Bordetella species, including B. pertussis, have a type III secretion system that is highly conserved among gram-negative pathogenic bacteria. Genes encoding the component proteins of the type III secretion system are localized at the bsc locus in the Bordetella genome. Here, the function of a hypothetical protein Bcr4 encoded at the bsc locus in the B. bronchiseptica genome was investigated. A Bcr4-deficient mutant was created and the amounts of type III secreted proteins (e.g., BopB, BopN and Bsp22) in both the supernatant fraction and whole-cell lysates of the Bcr4-deficient mutant were determined. It was found that the amounts of these proteins were significantly lower than in the wild-type strain. The amounts of type III secreted proteins in the supernatant fraction and whole-cell lysates were much greater in a Bcr4-overproducing strain than in the wild-type strain. The type III secreted protein BspR reportedly negatively regulates the type III secretion system. Here, it was observed that a Bcr4 + BspR double-knockout mutant did not secrete type III secreted proteins, whereas the amounts of these proteins in whole-cell lysates of this mutant were nearly equal to those in whole-cell lysates of the BspR-deficient mutant. Bcr4 thus appears to play an essential role in the extracellular secretion of type III secreted proteins. Our data also suggest that Bcr4 antagonizes the negative regulatory function of BspR.
Keywords: Bcr4; Bordetella; BspR; anti-sigma factor; type III secretion system.
© 2018 The Societies and John Wiley & Sons Australia, Ltd.
Similar articles
- Bcr4 Is a Chaperone for the Inner Rod Protein in the Bordetella Type III Secretion System.
Goto M, Abe A, Hanawa T, Suzuki M, Kuwae A. Goto M, et al. Microbiol Spectr. 2022 Oct 26;10(5):e0144322. doi: 10.1128/spectrum.01443-22. Epub 2022 Aug 30. Microbiol Spectr. 2022. PMID: 36040173 Free PMC article. - Bordetella Type III Secretion Injectosome and Effector Proteins.
Kamanova J. Kamanova J. Front Cell Infect Microbiol. 2020 Sep 4;10:466. doi: 10.3389/fcimb.2020.00466. eCollection 2020. Front Cell Infect Microbiol. 2020. PMID: 33014891 Free PMC article. Review. - BspR/BtrA, an Anti-σ Factor, Regulates the Ability of Bordetella bronchiseptica To Cause Cough in Rats.
Nakamura K, Shinoda N, Hiramatsu Y, Ohnishi S, Kamitani S, Ogura Y, Hayashi T, Horiguchi Y. Nakamura K, et al. mSphere. 2019 Apr 24;4(2):e00093-19. doi: 10.1128/mSphere.00093-19. mSphere. 2019. PMID: 31019000 Free PMC article. - Phosphorelay control of virulence gene expression in Bordetella.
Cotter PA, Jones AM. Cotter PA, et al. Trends Microbiol. 2003 Aug;11(8):367-73. doi: 10.1016/s0966-842x(03)00156-2. Trends Microbiol. 2003. PMID: 12915094 Review. No abstract available.
Cited by
- Bcr4 Is a Chaperone for the Inner Rod Protein in the Bordetella Type III Secretion System.
Goto M, Abe A, Hanawa T, Suzuki M, Kuwae A. Goto M, et al. Microbiol Spectr. 2022 Oct 26;10(5):e0144322. doi: 10.1128/spectrum.01443-22. Epub 2022 Aug 30. Microbiol Spectr. 2022. PMID: 36040173 Free PMC article. - Cyclic di-GMP Regulates the Type III Secretion System and Virulence in Bordetella bronchiseptica.
Gutierrez MP, Wong TY, Damron FH, Fernández J, Sisti F. Gutierrez MP, et al. Infect Immun. 2022 Jun 16;90(6):e0010722. doi: 10.1128/iai.00107-22. Epub 2022 May 25. Infect Immun. 2022. PMID: 35612302 Free PMC article. - Bordetella Type III Secretion Injectosome and Effector Proteins.
Kamanova J. Kamanova J. Front Cell Infect Microbiol. 2020 Sep 4;10:466. doi: 10.3389/fcimb.2020.00466. eCollection 2020. Front Cell Infect Microbiol. 2020. PMID: 33014891 Free PMC article. Review. - BspR/BtrA, an Anti-σ Factor, Regulates the Ability of Bordetella bronchiseptica To Cause Cough in Rats.
Nakamura K, Shinoda N, Hiramatsu Y, Ohnishi S, Kamitani S, Ogura Y, Hayashi T, Horiguchi Y. Nakamura K, et al. mSphere. 2019 Apr 24;4(2):e00093-19. doi: 10.1128/mSphere.00093-19. mSphere. 2019. PMID: 31019000 Free PMC article.
MeSH terms
Substances
Grants and funding
- 24390108 (to A.A.)/Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI
- 25670215 (A.A.)/Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI
- 16K08786 (A.A.)/Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI
- 26460535 (A.K.)/Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI
- 17K08838 (A.K.)/Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science KAKENHI
- Takeda Science Foundation
LinkOut - more resources
Full Text Sources