MicroRNA-98 attenuates cardiac ischemia-reperfusion injury through inhibiting DAPK1 expression - PubMed (original) (raw)

. 2019 Feb;71(2):166-176.

doi: 10.1002/iub.1879. Epub 2018 Nov 12.

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MicroRNA-98 attenuates cardiac ischemia-reperfusion injury through inhibiting DAPK1 expression

Chang-Lin Zhai et al. IUBMB Life. 2019 Feb.

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Abstract

Cardiovascular ischemic disease is a large class of diseases that are harmful to human health. The significant role of microRNAs (miRNAs) in terms of controlling cardiac injury has been reported in latest studies. MiR-98 is very important in regulating the apoptosis, the differentiation, the growth as well as the metastasis of cells. Nevertheless, the effect of miR-98 in the cardiac ischemia reperfusion (I/R) injury has rarely been investigated. In the current research, we found that the miR-98 expression was down-regulated in the cardiomyocytes subjected to hypoxia/reoxygenation (H/R) and in the myocardium of the I/R rats. In addition, over-expression of miR-98 could significantly reduce the myocardial oxidative stress and ischemic injury as well as cell apoptosis. In agreement, similar findings were demonstrated in H9c2 cells subjected to H/R injury. Bioinformatic analysis using MiRanda and TargetScan and luciferase activity assay confirmed death-associated protein kinase 1 (DAPK1) as a direct target of miR-98. These findings suggest that miR-98 may be exploited as a novel molecular marker or therapeutic target for myocardial I/R injury. © 2018 IUBMB Life, 71(1):166-176, 2019.

Keywords: DAPK1; hypoxia; ischemia-reperfusion injury; microRNA-98; myocardium.

© 2018 International Union of Biochemistry and Molecular Biology.

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