Enhancing carotenoid biosynthesis in rice endosperm by metabolic engineering - PubMed (original) (raw)
Enhancing carotenoid biosynthesis in rice endosperm. (a) Schematic representation of the recombinant vectors used for rice transformation. Upper:
PC
construction containing two genes; Lower:
HPC
construction containing three genes. (b) Western blot analyses of
PC
and
HPC
transgenic seed proteins. W, wild‐type; 1, 2 and 3, different lines with the
HPC
construction containing three genes; 4, 5 and 6, different lines with the
PC
construction containing two genes. (c) Genomic Southern blot analyses of
HPC
(1, 2) and
PC
(3, 4) transgenic rice plants.(d) Seed carotenoid content and composition of
PC
and
HPC
transgenic rice plants (μg/g dry weight) and comparison of representative seed colour phenotypes among nontransgenic wild‐type (W) rice and independent
PC
and
HPC
transgenic rice lines at the T4 generation. (e) Expression of endogenous carotenogenic genes in transgenic rice endosperm. Relative transcript levels of endogenous genes in T4 rice endosperms at 25
DAP
expressing different transgenic combinations. Values are the means ±
SD
of three quantitative real‐time
PCR
replicates. (f) The agronomic traits of
PC
(1,2,3) and
HPC
(4,5,6) transgenic rice plants. (g) Scanning electron microscope images of transverse sections of mature rice seeds. W: wild‐type;
PC
: expressing ZmPSY1 and PaCRTI;
HPC
: expressing tHMG1, ZmPSY1 and PaCRTI. Scale bars: 50 μm.