Enhancing carotenoid biosynthesis in rice endosperm by metabolic engineering - PubMed (original) (raw)

Enhancing carotenoid biosynthesis in rice endosperm. (a) Schematic representation of the recombinant vectors used for rice transformation. Upper:

PC

construction containing two genes; Lower:

HPC

construction containing three genes. (b) Western blot analyses of

PC

and

HPC

transgenic seed proteins. W, wild‐type; 1, 2 and 3, different lines with the

HPC

construction containing three genes; 4, 5 and 6, different lines with the

PC

construction containing two genes. (c) Genomic Southern blot analyses of

HPC

(1, 2) and

PC

(3, 4) transgenic rice plants.(d) Seed carotenoid content and composition of

PC

and

HPC

transgenic rice plants (μg/g dry weight) and comparison of representative seed colour phenotypes among nontransgenic wild‐type (W) rice and independent

PC

and

HPC

transgenic rice lines at the T4 generation. (e) Expression of endogenous carotenogenic genes in transgenic rice endosperm. Relative transcript levels of endogenous genes in T4 rice endosperms at 25

DAP

expressing different transgenic combinations. Values are the means ±

SD

of three quantitative real‐time

PCR

replicates. (f) The agronomic traits of

PC

(1,2,3) and

HPC

(4,5,6) transgenic rice plants. (g) Scanning electron microscope images of transverse sections of mature rice seeds. W: wild‐type;

PC

: expressing ZmPSY1 and PaCRTI;

HPC

: expressing tHMG1, ZmPSY1 and PaCRTI. Scale bars: 50 μm.