Oligodendrocytes and CNS myelin are nonpermissive substrates for neurite growth and fibroblast spreading in vitro - PubMed (original) (raw)
Oligodendrocytes and CNS myelin are nonpermissive substrates for neurite growth and fibroblast spreading in vitro
M E Schwab et al. J Neurosci. 1988 Jul.
Abstract
To study the interaction of neurons with CNS glial cells, dissociated sympathetic or sensory ganglion cells or fetal retinal cells were plated onto cultures of dissociated optic nerve glial cells of young rats. Whereas astrocytes favored neuron adhesion and neurite outgrowth, oligodendrocytes differed markedly in their properties as neuronal substrates. Immature (O4+, A2B5+, GalC-) oligodendrocytes were frequently contacted by neurons and neurites. In contrast, differentiated oligodendrocytes (O4+, A2B5-, GalC+) represented a nonpermissive substrate for neuronal adhesion and neurite growth. When neuroblastoma cells or 3T3 fibroblasts were plated into optic nerve glial cultures, the same differences were observed; differentiated oligodendrocytes were nonpermissive for cell adhesion, neurite growth, or fibroblast spreading. These nonpermissive oligodendrocytes were characterized by a radial, highly branched process network, often contained myelin basic protein, and may, therefore, correspond to cells actively involved in the production of myelin-like membranes. Isolated myelin from adult rat spinal cord was adsorbed to polylysine-coated culture dishes and tested as a substrate for peripheral neurons, neuroblastoma cells, or 3T3 cells. Again, cell attachment, neurite outgrowth, and fibroblast spreading was strongly impaired. General physicochemical properties of myelin were not responsible for this effect, since myelin from rat sciatic nerves favored neuron adhesion and neurite growth as well as spreading of 3T3 cells. These results show that differentiated oligodendrocytes express nonpermissive substrate properties, which may be of importance in CNS development or regeneration.
Similar articles
- Retinal axon regeneration in the lizard Gallotia galloti in the presence of CNS myelin and oligodendrocytes.
Lang DM, Monzón-Mayor M, Bandtlow CE, Stuermer CA. Lang DM, et al. Glia. 1998 May;23(1):61-74. doi: 10.1002/(sici)1098-1136(199805)23:1<61::aid-glia6>3.0.co;2-7. Glia. 1998. PMID: 9562185 - Two membrane protein fractions from rat central myelin with inhibitory properties for neurite growth and fibroblast spreading.
Caroni P, Schwab ME. Caroni P, et al. J Cell Biol. 1988 Apr;106(4):1281-8. doi: 10.1083/jcb.106.4.1281. J Cell Biol. 1988. PMID: 3360853 Free PMC article. - Oligodendrocyte- and myelin-associated inhibitors of neurite outgrowth: their involvement in the lack of CNS regeneration.
Cadelli DS, Bandtlow CE, Schwab ME. Cadelli DS, et al. Exp Neurol. 1992 Jan;115(1):189-92. doi: 10.1016/0014-4886(92)90246-m. Exp Neurol. 1992. PMID: 1728566 - Central nervous system regeneration: oligodendrocytes and myelin as non-permissive substrates for neurite growth.
Caroni P, Savio T, Schwab ME. Caroni P, et al. Prog Brain Res. 1988;78:363-70. doi: 10.1016/s0079-6123(08)60305-2. Prog Brain Res. 1988. PMID: 3073419 Review. No abstract available. - Myelin-associated inhibitors of neurite growth.
Schwab ME. Schwab ME. Exp Neurol. 1990 Jul;109(1):2-5. doi: 10.1016/s0014-4886(05)80003-2. Exp Neurol. 1990. PMID: 2192907 Review.
Cited by
- Microglial Phagocytosis During Embryonic and Postnatal Development.
Marín-Teva JL, Sepúlveda MR, Neubrand VE, Cuadros MA. Marín-Teva JL, et al. Adv Neurobiol. 2024;37:151-161. doi: 10.1007/978-3-031-55529-9_9. Adv Neurobiol. 2024. PMID: 39207691 Review. - Neural regeneration in the human central nervous system-from understanding the underlying mechanisms to developing treatments. Where do we stand today?
Kvistad CE, Kråkenes T, Gavasso S, Bø L. Kvistad CE, et al. Front Neurol. 2024 May 9;15:1398089. doi: 10.3389/fneur.2024.1398089. eCollection 2024. Front Neurol. 2024. PMID: 38803647 Free PMC article. Review. - Myelination-independent functions of oligodendrocyte precursor cells in health and disease.
Xiao Y, Czopka T. Xiao Y, et al. Nat Neurosci. 2023 Oct;26(10):1663-1669. doi: 10.1038/s41593-023-01423-3. Epub 2023 Aug 31. Nat Neurosci. 2023. PMID: 37653126 Review. - Toward reframing brain-social dynamics: current assumptions and future challenges.
Faraji J, Metz GAS. Faraji J, et al. Front Psychiatry. 2023 Jul 6;14:1211442. doi: 10.3389/fpsyt.2023.1211442. eCollection 2023. Front Psychiatry. 2023. PMID: 37484686 Free PMC article. Review. - Roles and regulation of microglia activity in multiple sclerosis: insights from animal models.
Distéfano-Gagné F, Bitarafan S, Lacroix S, Gosselin D. Distéfano-Gagné F, et al. Nat Rev Neurosci. 2023 Jul;24(7):397-415. doi: 10.1038/s41583-023-00709-6. Epub 2023 Jun 2. Nat Rev Neurosci. 2023. PMID: 37268822 Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources