A labile inhibitor blocks immunoglobulin kappa-light-chain-gene transcription in a pre-B leukemic cell line - PubMed (original) (raw)

A labile inhibitor blocks immunoglobulin kappa-light-chain-gene transcription in a pre-B leukemic cell line

R Wall et al. Proc Natl Acad Sci U S A. 1986 Jan.

Abstract

The murine pre-B leukemic cell line 70Z/3 contains both an unrearranged immunoglobulin kappa-light-chain gene and a functionally rearranged but silent kappa-light-chain gene. Mitogenic stimulation of growing 70Z/3 cells with bacterial lipopolysaccharide (LPS) activates kappa-light-gene transcription and results in a 10- to 20-fold increase in cytoplasmic kappa-light-chain mRNA. The induction of kappa gene expression by LPS was probed by using an inhibitor of protein synthesis. Concomitant treatment of 70Z/3 cells with LPS and cycloheximide failed to block kappa-light-chain mRNA accumulation, indicating that new protein synthesis is not required for the activation of kappa-gene expression. Treatment of 70Z/3 cells with cycloheximide alone resulted in kappa-mRNA induction equivalent to those produced by LPS alone. The kappa mRNA synthesized in the presence of cycloheximide was intact and able to direct the synthesis of kappa light chains. Nuclear transcription assays revealed that cycloheximide, like LPS, activated kappa-gene transcription. These findings indicate that the trans-acting factors necessary for kappa-light-chain-gene transcription are present in pre-B cells, but their activity is blocked by short-lived inhibitory proteins.

PubMed Disclaimer

Similar articles

• Induction of kappa transcription by interferon-gamma without activation of NF-kappa B.
  Briskin M, Kuwabara MD, Sigman DS, Wall R. Briskin M, et al. Science. 1988 Nov 18;242(4881):1036-7. doi: 10.1126/science.3143155. Science. 1988. PMID: 3143155
• Lipopolysaccharide-unresponsive mutant pre-B-cell lines blocked in NF-kappa B activation.
  Briskin M, Damore M, Law R, Lee G, Kincade PW, Sibley CH, Kuehl M, Wall R. Briskin M, et al. Mol Cell Biol. 1990 Jan;10(1):422-5. doi: 10.1128/mcb.10.1.422-425.1990. Mol Cell Biol. 1990. PMID: 2104663 Free PMC article.
• Inducible transcription of the unrearranged kappa constant region locus is a common feature of pre-B cells and does not require DNA or protein synthesis.
  Nelson KJ, Kelley DE, Perry RP. Nelson KJ, et al. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5305-9. doi: 10.1073/pnas.82.16.5305. Proc Natl Acad Sci U S A. 1985. PMID: 3927301 Free PMC article.
• Interleukin-4 inhibits kappa light chain expression and NF kappa B activation but not I kappa B alpha degradation in 70Z/3 murine pre-B cells.
  Clarke CJ, Taylor-Fishwick DA, Hales A, Chernajovsky Y, Sugamura K, Feldmann M, Foxwell BM. Clarke CJ, et al. Eur J Immunol. 1995 Oct;25(10):2961-6. doi: 10.1002/eji.1830251037. Eur J Immunol. 1995. PMID: 7589098
• Slow response variant of the B lymphoma 70Z/3 defective in LPS activation of NF-kappa B.
  Rooney JW, Emery DW, Sibley CH. Rooney JW, et al. Immunogenetics. 1990;31(2):65-72. doi: 10.1007/BF00661215. Immunogenetics. 1990. PMID: 2105908

Cited by

• The origins of NF-κB.
  Sen R. Sen R. Nat Immunol. 2011 Jul 19;12(8):686-8. doi: 10.1038/ni.2071. Nat Immunol. 2011. PMID: 21772276 No abstract available.
• Protein synthesis inhibitors, gene superinduction and memory: too little or too much protein?
  Radulovic J, Tronson NC. Radulovic J, et al. Neurobiol Learn Mem. 2008 Mar;89(3):212-8. doi: 10.1016/j.nlm.2007.08.008. Epub 2007 Sep 29. Neurobiol Learn Mem. 2008. PMID: 17904877 Free PMC article. Review.
• Plural light chains in a single plasma cell of a monoclonal gammopathy undetermined significance case: an ultrastructural study.
  Saito N, Konishi K, Ohta S, Kondo T, Kato M, Hashino S, Takeda H, Asaka M, Ooi HK. Saito N, et al. Hum Cell. 2007 Feb;20(1):10-4. doi: 10.1111/j.1749-0774.2007.00026.x. Hum Cell. 2007. PMID: 17506772
• Enhancement of antigen-presenting ability of B lymphoma cells by partial inhibition of protein synthesis through inducing B7-1 expression.
  Aoi T, Nakano H, Tanaka Y, Kakiuchi T. Aoi T, et al. Immunology. 1997 Jun;91(2):212-8. doi: 10.1046/j.1365-2567.1997.00253.x. Immunology. 1997. PMID: 9227319 Free PMC article.
• Negative transcriptional regulation of human interleukin 2 (IL-2) gene by glucocorticoids through interference with nuclear transcription factors AP-1 and NF-AT.
  Paliogianni F, Raptis A, Ahuja SS, Najjar SM, Boumpas DT. Paliogianni F, et al. J Clin Invest. 1993 Apr;91(4):1481-9. doi: 10.1172/JCI116353. J Clin Invest. 1993. PMID: 8473495 Free PMC article.

References

1. 1. Nature. 1984 Aug 23-29;310(5979):697-8 - PubMed
2. 1. J Virol. 1983 Feb;45(2):683-92 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7476-80 - PubMed
4. 1. J Exp Med. 1984 Dec 1;160(6):1937-42 - PubMed
5. 1. Nature. 1985 Jul 25-31;316(6026):356-8 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database