GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae - PubMed (original) (raw)
GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae
J F Tschopp et al. J Bacteriol. 1986 Apr.
Abstract
The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.
Similar articles
- The yeast IMP1 gene is allelic to GAL2.
Ulery TL, Mangus DA, Jaehning JA. Ulery TL, et al. Mol Gen Genet. 1991 Nov;230(1-2):129-35. doi: 10.1007/BF00290660. Mol Gen Genet. 1991. PMID: 1745225 - Yeast galactose permease is related to yeast and mammalian glucose transporters.
Nehlin JO, Carlberg M, Ronne H. Nehlin JO, et al. Gene. 1989 Dec 28;85(2):313-9. doi: 10.1016/0378-1119(89)90423-x. Gene. 1989. PMID: 2697639 - Construction of strains of Saccharomyces cerevisiae that grow on lactose.
Sreekrishna K, Dickson RC. Sreekrishna K, et al. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7909-13. doi: 10.1073/pnas.82.23.7909. Proc Natl Acad Sci U S A. 1985. PMID: 3934664 Free PMC article. - Nucleosome transactions on the promoters of the yeast GAL and PHO genes.
Lohr D. Lohr D. J Biol Chem. 1997 Oct 24;272(43):26795-8. doi: 10.1074/jbc.272.43.26795. J Biol Chem. 1997. PMID: 9341105 Review. No abstract available. - Multiple or pleiotropic drug resistance in yeast.
Balzi E, Goffeau A. Balzi E, et al. Biochim Biophys Acta. 1991 Mar 4;1073(2):241-52. doi: 10.1016/0304-4165(91)90128-4. Biochim Biophys Acta. 1991. PMID: 2009277 Review. No abstract available.
Cited by
- Evolution and functional diversification of yeast sugar transporters.
Donzella L, Sousa MJ, Morrissey JP. Donzella L, et al. Essays Biochem. 2023 Sep 13;67(5):811-827. doi: 10.1042/EBC20220233. Essays Biochem. 2023. PMID: 36928992 Free PMC article. - Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression.
Griggs DW, Johnston M. Griggs DW, et al. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8597-601. doi: 10.1073/pnas.88.19.8597. Proc Natl Acad Sci U S A. 1991. PMID: 1924319 Free PMC article. - Three galactose inducible promoters for use in C. neoformans var. grubii.
Ruff JA, Lodge JK, Baker LG. Ruff JA, et al. Fungal Genet Biol. 2009 Jan;46(1):9-16. doi: 10.1016/j.fgb.2008.10.003. Epub 2008 Oct 14. Fungal Genet Biol. 2009. PMID: 18952189 Free PMC article. - Biofuel Production Based on Carbohydrates from Both Brown and Red Macroalgae: Recent Developments in Key Biotechnologies.
Kawai S, Murata K. Kawai S, et al. Int J Mol Sci. 2016 Feb 6;17(2):145. doi: 10.3390/ijms17020145. Int J Mol Sci. 2016. PMID: 26861307 Free PMC article. Review. - Sequence and structure of the yeast galactose transporter.
Szkutnicka K, Tschopp JF, Andrews L, Cirillo VP. Szkutnicka K, et al. J Bacteriol. 1989 Aug;171(8):4486-93. doi: 10.1128/jb.171.8.4486-4493.1989. J Bacteriol. 1989. PMID: 2666404 Free PMC article.
References
- J Bacteriol. 1970 Sep;103(3):671-8 - PubMed
- EMBO J. 1984 Dec 20;3(13):3157-64 - PubMed
- Nature. 1978 Sep 14;275(5676):104-9 - PubMed
- Cell. 1980 Mar;19(3):753-64 - PubMed
- Cell. 1980 Aug;21(1):205-15 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials