GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae - PubMed (original) (raw)

GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae

J F Tschopp et al. J Bacteriol. 1986 Apr.

Abstract

The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.

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References

    1. J Bacteriol. 1970 Sep;103(3):671-8 - PubMed
    1. EMBO J. 1984 Dec 20;3(13):3157-64 - PubMed
    1. Nature. 1978 Sep 14;275(5676):104-9 - PubMed
    1. Cell. 1980 Mar;19(3):753-64 - PubMed
    1. Cell. 1980 Aug;21(1):205-15 - PubMed

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