Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences - PubMed (original) (raw)

Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences

A Alvarez-Morales et al. Nucleic Acids Res. 1986.

Free PMC article

Abstract

Previous analysis of B. japonicum nifH'- and nifD'-'lacZ translational fusions showed that these promoters could be activated by the K. pneumoniae nifA plus the E. coli ntrA gene products. To study the functions of the DNA 5' to these promoters, plasmids carrying deletions in this region were constructed and analyzed in vivo in a heterologous system consisting of an E. coli (NtrA+) background with a plasmid that constitutively expresses the K. pneumoniae nifA gene. Activation of the B. japonicum promoters was completely dependent on sequences located between positions -165 and -100, relative to the start of transcription. Some of the nifD deletion-fusions were mobilized to the wild-type B. japonicum and the exconjugants tested in an ex planta micro-aerobic system, and also used to infect soybean seedlings. The time course of derepression was followed by assaying beta-galactosidase activity from samples withdrawn from the microaerobic cultures or from root-nodule extracts. The results conclusively show that in the homologous system the sequences upstream of the promoter are required to achieve wild-type activity.

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References

1. EMBO J. 1986 Feb;5(2):441-7 - PubMed
1. J Bacteriol. 1984 Jun;158(3):1005-11 - PubMed
1. Nucleic Acids Res. 1984 Sep 25;12(18):7123-34 - PubMed
1. Nucleic Acids Res. 1985 May 24;13(10):3407-18 - PubMed
1. J Bacteriol. 1983 Dec;156(3):1025-34 - PubMed

Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences - PubMed (original) (raw)