Abscisic acid is a substrate of the ABC transporter encoded by the durable wheat disease resistance gene Lr34 - PubMed (original) (raw)

LR

34 transports abscisic acid (

ABA

). (a) Rice blast resistance and leaf tip necrosis in 4‐wk‐old plants of _Lr34res‐_expressing line 19, _Lr34sus‐_expressing line 131 and 19sib. (b) Tritiated abscisic acid (3H‐

ABA

) accumulation (disintegrations per minute, d.p.m.) in rice seedlings of lines 11, 19, and 131 compared with respective sib lines. N = 3, n = 13–23, mean ±

SEM

. *, P < 0.01 compared with sib (Student's _t_‐test). (c) Relative 3H‐

ABA

accumulation in yeast strain W303 expressing Lr34res or Lr34sus. Mean ±

SE

, **, P < 0.01 compared with empty vector (

EV

) control. The graphs show averaged results of three independent experiments. (d) Relative 3H‐

ABA

accumulation in yeast strain

YMM

12 expressing Lr34res or Lr34sus. Mean ±

SE

, **, P < 0.01 compared with

EV

control. The graphs show averaged results of three independent experiments. (e) Relative 3H‐

ABA

accumulation in yeast strain

YMM

12 after preincubation with the

ABC

transporter inhibitor glibenclamide (

GC

) and the

ABCC

‐specific inhibitor probenecid (Pro). The control (Con) without inhibitor was set to 100%, mean ±

SE

. *, P < 0.05, **, P < 0.01. (f) Relative 3H‐

ABA

accumulation (50

nM

) in yeast strain

YMM

12 in the absence (Con) or presence of cold

ABA

. The control (Con) without inhibitor was set to 100%, mean ±

SEM

. *, P < 0.05, **, P < 0.01.