High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA - PubMed (original) (raw)

High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA

D Simon et al. Appl Environ Microbiol. 1986 Aug.

Abstract

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).

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References

1. 1. Appl Environ Microbiol. 1984 Aug;48(2):347-51 -PubMed
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