Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness - PubMed (original) (raw)

Immune responses during human Schistosomiasis mansoni. Evidence for antiidiotypic T lymphocyte responsiveness

M S Lima et al. J Clin Invest. 1986 Oct.

Abstract

We present a method for the examination of antiidiotypic cell-mediated reactivity during chronic human infections. Pooled and individual sera from patients with schistosomiasis mansoni were purified on immunoaffinity columns of schistosomal egg antigens (SEA). The eluates contained anti-SEA antibodies, but not SEA. These antibody preparations, and their F(ab)2 fragments, stimulated dose-dependent proliferation of peripheral blood mononuclear cells (PBMN) and T lymphocytes from some, but not all active or former schistosomiasis mansoni patients, and could do so autologously. Stimulation required presentation by plastic-adherent cells. The eluates did not stimulate PBMN from persons who had never had schistosomiasis. Affinity-purified anti-SEA antibodies from former patients (cured for greater than 10 yr) did not stimulate PBMN from patients with active infections. Reabsorption on SEA columns removed stimulatory activity from the eluates. We propose that multiclonal, SEA-related idiotypes expressed by some anti-SEA antibodies stimulate proliferation of T lymphocytes that express antiidiotypic specificities.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Immunol. 1984 Aug;133(2):993-7 - PubMed
    1. Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397-400 - PubMed
    1. Ann Immunol (Paris). 1974 Jan;125C(1-2):373-89 - PubMed
    1. J Exp Med. 1976 Mar 1;143(3):696-700 - PubMed

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