Recombinant human interferon-gamma inhibits formation of human osteoclast-like cells - PubMed (original) (raw)
. 1986 Dec 1;137(11):3544-9.
- PMID: 3097126
Recombinant human interferon-gamma inhibits formation of human osteoclast-like cells
N Takahashi et al. J Immunol. 1986.
Abstract
Interferon-gamma (IFN-gamma) inhibits osteoclastic bone resorption in vitro, but the mechanism responsible for this inhibition is unknown. We have used a long-term human marrow culture system that forms multinucleated cells (MNC) with osteoclast characteristics to test the effect of recombinant human IFN-gamma on MNC formation. The addition of 1,25-dihydroxy-vitamin D3 (1,25D3) at 10(-8) M to these cultures significantly increased both MNC formation and the number of nuclei per MNC. IFN-gamma at 100 U/ml strongly inhibited both of these effects of 1,25D3 in this system. IFN-gamma significantly inhibited MNC formation at very low concentrations (4 U/ml), with 10 U/ml inhibiting 1,25D3-stimulated MNC formation by 50%. In contrast, 100 U/ml of IFN-gamma were required to inhibit the growth of granulocyte-macrophage colony-forming cells, the probable progenitor for MNC, by 50%. Treatment of cultures with IFN-gamma for only the first or last week of culture significantly inhibited MNC formation stimulated by 1,25D3. Autoradiographic studies with [3H]thymidine showed that IFN-gamma did not inhibit proliferation of precursors for MNC. Additionally, IFN-gamma inhibited MNC formation stimulated by parathyroid hormone or interleukin 1. These results suggest that IFN-gamma inhibits MNC formation, and that IFN-gamma inhibits bone resorption in part by inhibiting osteoclast formation.
Similar articles
- Osteoclast-like cell formation in fetal and newborn long-term baboon marrow cultures is more sensitive to 1,25-dihydroxyvitamin D3 than adult long-term marrow cultures.
Takahashi N, Mundy GR, Kuehl TJ, Roodman GD. Takahashi N, et al. J Bone Miner Res. 1987 Aug;2(4):311-7. doi: 10.1002/jbmr.5650020408. J Bone Miner Res. 1987. PMID: 3455616 - Interleukin-1 and tumor necrosis factor stimulate the formation of human osteoclastlike cells in vitro.
Pfeilschifter J, Chenu C, Bird A, Mundy GR, Roodman GD. Pfeilschifter J, et al. J Bone Miner Res. 1989 Feb;4(1):113-8. doi: 10.1002/jbmr.5650040116. J Bone Miner Res. 1989. PMID: 2785743 - Interferons-alpha and -gamma inhibit interleukin-1 beta-stimulated osteoclast-like cell formation in long-term human marrow cultures.
Kurihara N, Roodman GD. Kurihara N, et al. J Interferon Res. 1990 Oct;10(5):541-7. doi: 10.1089/jir.1990.10.541. J Interferon Res. 1990. PMID: 2125633 - Immune cells and bone resorption.
Gowen M, MacDonald BR, Hughes DE, Skjodt H, Russell RG. Gowen M, et al. Adv Exp Med Biol. 1986;208:261-73. doi: 10.1007/978-1-4684-5206-8_33. Adv Exp Med Biol. 1986. PMID: 3105268 Review. No abstract available. - The origin of transformed cells. studies of spontaneous and induced cell transformation in cell cultures from marsupials, a snail, and human amniocytes.
Walen KH. Walen KH. Cancer Genet Cytogenet. 2002 Feb;133(1):45-54. doi: 10.1016/s0165-4608(01)00572-6. Cancer Genet Cytogenet. 2002. PMID: 11890989 Review.
Cited by
- Osteoporosis: interferon-gamma-mediated bone remodeling in osteoimmunology.
Li S, Liu G, Hu S. Li S, et al. Front Immunol. 2024 May 16;15:1396122. doi: 10.3389/fimmu.2024.1396122. eCollection 2024. Front Immunol. 2024. PMID: 38817601 Free PMC article. Review. - 3D Organoids for Regenerative Endodontics.
Li FC, Kishen A. Li FC, et al. Biomolecules. 2023 May 28;13(6):900. doi: 10.3390/biom13060900. Biomolecules. 2023. PMID: 37371480 Free PMC article. Review. - Inflammatory activation of the FcγR and IFNγR pathways co-influences the differentiation and activity of osteoclasts.
Groetsch B, Schachtschabel E, Tripal P, Schmid B, Smith AS, Schett G, Bozec A. Groetsch B, et al. Front Immunol. 2022 Sep 6;13:958974. doi: 10.3389/fimmu.2022.958974. eCollection 2022. Front Immunol. 2022. PMID: 36148242 Free PMC article. - The promising roles of macrophages in geriatric hip fracture.
Lu YN, Wang L, Zhang YZ. Lu YN, et al. Front Cell Dev Biol. 2022 Aug 26;10:962990. doi: 10.3389/fcell.2022.962990. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36092716 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources