Monoclonal origin of B lymphocyte colony-forming cells in spleen colonies formed by multipotential hemopoietic stem cells - PubMed (original) (raw)
Monoclonal origin of B lymphocyte colony-forming cells in spleen colonies formed by multipotential hemopoietic stem cells
P K Lala et al. J Exp Med. 1978.
Abstract
Spleen colonies produced by transplanting lethally irradiated mice with either 12 day fetal liver or adult bone marrow cells were found to contain B- lymphocyte colony-forming cells (BL-CFC) . The proportion of BL-CFC positive spleen colonies did not increase substantially between 8 and 14 days after transplantation, the range being 18-45 percent. However, the absolute number of BL-CFC per spleen colony varied considerably (between 1 and 10,318), although the majority of colonies contained less than 200 BL-CFC. Irrespective of the time after transplantation, smaller spleen colonies were found to have a higher frequency of BL-CFC than larger spleen colonies. To determine the possible clonal origin of BL-CFC from spleen colony- forming unit (CFU-S), CBA mice were injected with equal numbers of CBA and CBA T(6)/T(6) fetal liver or adult bone marrow cells. Analysis of 7-15-day spleen colonies demonstrated that 90 percent were either exclusively T(6) positive or T(6) negative and approximately equal numbers ofboth colony types were observed. B-lymphocyte colonies were grown and successfully karyotyped from 19 spleen colonies. When compared with the original spleen colony karyotype the B-lymphocyte colony cells karyotype was identical in all 19 cases. In 3 of the 19 colonies analyzed a mixture of T(6) positive and T(6) negative karyotypes was present and identical proportions of the karyotypes were present in the pooled B-lymphocyte colony cells and spleen colony cells. The data indicate that the B-lymphocyte colony-forming cells detected in spleen colonies are genuine members of the hemopoietic clone derived from the initiating hemopoietic stem cell (CFU-S).
Similar articles
- Colony formation in agar by adult bone marrow multipotential hemopoietic cells.
Johnson GR. Johnson GR. J Cell Physiol. 1980 Jun;103(3):371-83. doi: 10.1002/jcp.1041030302. J Cell Physiol. 1980. PMID: 7400223 - Characterization of two populations of CFU-S fractionated from mouse fetal liver by fluorescence-activated cell sorting.
Johnson GR, Nicola NA. Johnson GR, et al. J Cell Physiol. 1984 Jan;118(1):45-52. doi: 10.1002/jcp.1041180110. J Cell Physiol. 1984. PMID: 6690451 - Colony formation in agar by multipotential hemopoietic cells.
Metcalf D, Johnson GR, Mandel TE. Metcalf D, et al. J Cell Physiol. 1979 Feb;98(2):401-20. doi: 10.1002/jcp.1040980216. J Cell Physiol. 1979. PMID: 422666 - Formation of B lymphocytes in fetal and adult life.
Kincade PW. Kincade PW. Adv Immunol. 1981;31:177-245. doi: 10.1016/s0065-2776(08)60921-9. Adv Immunol. 1981. PMID: 7032254 Review. No abstract available.
Cited by
- Murine B-lymphocyte colony formation: the effects of cyclophosphamide and azathioprine.
Winkelstein A. Winkelstein A. Immunology. 1982 Aug;46(4):827-32. Immunology. 1982. PMID: 6980824 Free PMC article. - The fate of fetal and adult B-cell progenitors grafted into immunodeficient CBA/N mice.
Paige CJ, Kincade PW, Moore MA, Lee G. Paige CJ, et al. J Exp Med. 1979 Sep 19;150(3):548-63. doi: 10.1084/jem.150.3.548. J Exp Med. 1979. PMID: 39107 Free PMC article. - Precursors of murine B lymphocytes. Physical and functional characterization, and distinctions from myeloid stem cells.
Paige CJ, Kincade PW, Shinefeld LA, Sato VL. Paige CJ, et al. J Exp Med. 1981 Jan 1;153(1):154-65. doi: 10.1084/jem.153.1.154. J Exp Med. 1981. PMID: 6969781 Free PMC article. - Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.
de Vries P, Brasel KA, McKenna HJ, Williams DE, Watson JD. de Vries P, et al. J Exp Med. 1992 Dec 1;176(6):1503-9. doi: 10.1084/jem.176.6.1503. J Exp Med. 1992. PMID: 1281208 Free PMC article.
References
- Radiat Res. 1961 Feb;14:213-22 - PubMed
- Stain Technol. 1958 Mar;33(2):73-7 - PubMed
- J Exp Med. 1977 Jun 1;145(6):1567-79 - PubMed
- J Immunol. 1977 Jun;118(6):2067-72 - PubMed
- Immunology. 1976 Jun;30(6):907-14 - PubMed