Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes - PubMed (original) (raw)
Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes
S M Fu et al. J Exp Med. 1978.
Abstract
Successful induction of in vitro differentiation and immunoglobulin synthesis of the leukemic lymphocytes was carried out in two cases of chronic lymphocytic leukemia. Few plasma cells and little specific Ig secretion were detected in the cultures of isolated leukemic B cells in either the presence or the absence of autologous T cells. Up to 30% of the leukemic B cells matured to plasma cells, and a 32-fold increase in specific Ig synthesis was observed when T cells from normal individuals were added to the cultures of these leukemic B cells. In one of the two cases, autologous T cells were able to induce greater than 50% of the leukemic B cells to differentiate further to plasma cells in the presence of pokeweed mitogen. This markedly accelerated in vitro differentiation was only achieved with leukemic cells from cases in which there was evidence of slight differentiation in vivo. No evidence could be obtained for excessive suppressor T cells in these patients. However, a T-cell defect in the generation of allogeneic effect helper factors was identified. This defect may be responsible for the reduced rate of leukemic maturation in vivo.
Similar articles
- Characterization of a suppressor-cell leukemia. Evidence for the requirement of an interaction of two T cells in the development of human suppressor effector cells.
Broder S, Poplack D, Whang-Peng J, Durm M, Goldman C, Muul L, Waldmann TA. Broder S, et al. N Engl J Med. 1978 Jan 12;298(2):66-72. doi: 10.1056/NEJM197801122980202. N Engl J Med. 1978. PMID: 304175 - In vitro induction of IgM secretion and switching to IgG production in human B leukemic cells with the help of T cells.
Saiki O, Kishimoto T, Kuritani T, Muraguchi A, Yamamura Y. Saiki O, et al. J Immunol. 1980 Jun;124(6):2609-14. J Immunol. 1980. PMID: 6966294 - Differentiation capacity and other properties of the leukemic cells of chronic lymphocytic leukemia.
Fu SM, Chiorazzi N, Kunkel HG. Fu SM, et al. Immunol Rev. 1979;48:23-44. doi: 10.1111/j.1600-065x.1979.tb00297.x. Immunol Rev. 1979. PMID: 232686 Review. No abstract available. - Regulation of the humoral immune response: from immunoglobulin genes to regulatory T cell networks.
Waldmann TA, Korsmeyer SJ, Hieter PA, Ravetch JV, Broder S, Leder P. Waldmann TA, et al. Fed Proc. 1983 May 15;42(8):2498-503. Fed Proc. 1983. PMID: 6404657 Review.
Cited by
- Differentiation of chronic lymphocytic leukemia B cells into immunoglobulin secreting cells decreases LEF-1 expression.
Gutierrez A Jr, Arendt BK, Tschumper RC, Kay NE, Zent CS, Jelinek DF. Gutierrez A Jr, et al. PLoS One. 2011;6(10):e26056. doi: 10.1371/journal.pone.0026056. Epub 2011 Oct 6. PLoS One. 2011. PMID: 21998751 Free PMC article. - Modulation of synovial cell products by a factor from a human cell line: T lymphocyte induction of a mononuclear cell factor.
Amento EP, Kurnick JT, Epstein A, Krane SM. Amento EP, et al. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5307-11. doi: 10.1073/pnas.79.17.5307. Proc Natl Acad Sci U S A. 1982. PMID: 6982471 Free PMC article. - Chronic lymphocytic leukemia cells diversify and differentiate in vivo via a nonclassical Th1-dependent, Bcl-6-deficient process.
Patten PE, Ferrer G, Chen SS, Simone R, Marsilio S, Yan XJ, Gitto Z, Yuan C, Kolitz JE, Barrientos J, Allen SL, Rai KR, MacCarthy T, Chu CC, Chiorazzi N. Patten PE, et al. JCI Insight. 2016 Apr 7;1(4):e86288. doi: 10.1172/jci.insight.86288. JCI Insight. 2016. PMID: 27158669 Free PMC article. - Differential mechanism for differentiation into immunoglobulin-secreting cells in human resting B lymphocyte subsets isolated on the basis of cell density.
Suzuki N, Ueda Y, Sakane T. Suzuki N, et al. J Clin Invest. 1988 Jan;81(1):261-9. doi: 10.1172/JCI113304. J Clin Invest. 1988. PMID: 2826542 Free PMC article. - Spontaneous in vitro differentiation of antigen-specific lymphocytes from a patient with immunoglobulin M gammopathy.
Halper J, Kabat EA, Osserman EF, Tonda P, Pernis B. Halper J, et al. J Clin Invest. 1982 Dec;70(6):1292-9. doi: 10.1172/jci110728. J Clin Invest. 1982. PMID: 6983530 Free PMC article.
References
- J Immunol. 1977 Mar;118(3):742-8 - PubMed
- J Immunol. 1977 Jun;118(6):2281-7 - PubMed
- J Immunol. 1978 Jul;121(1):1-5 - PubMed
- J Immunol. 1978 May;120(5):1480-4 - PubMed
- J Exp Med. 1976 Sep 1;144(3):674-84 - PubMed