A newly isolated human intestinal bacterium strain capable of deglycosylating flavone C-glycosides and its functional properties - PubMed (original) (raw)

A newly isolated human intestinal bacterium strain capable of deglycosylating flavone C-glycosides and its functional properties

Shiqi Zheng et al. Microb Cell Fact. 2019.

Abstract

Background: Flavone C-glycosides are difficult to be deglycosylated using traditional chemical methods due to their solid carbon-carbon bond between sugar moieties and aglycones; however, some bacteria may easily cleave this bond because they generate various specific enzymes.

Results: A bacterial strain, named W12-1, capable of deglycosylating orientin, vitexin, and isovitexin to their aglycones, was isolated from human intestinal bacteria in this study and identified as Enterococcus faecalis based on morphological examination, physiological and biochemical identification, and 16S rDNA sequencing. The strain was shown to preferentially deglycosylate the flavone C-glycosides on condition that the culture medium was short of carbon nutrition sources such as glucose and starch, and its deglycosylation efficiency was negatively correlated with the content of the latter two substances.

Conclusion: This study provided a new bacterial resource for the cleavage of C-glycosidic bond of flavone C-glycosides and reported the carbon nutrition sources reduction induced deglycosylation for the first time.

Keywords: Deglycosylation; Enterococcus faecalis; Flavone C-glycosides; Reduced carbon nutrition source induction.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1

Fig. 1

Structures and transformation of the compounds of interest

Fig. 2

Fig. 2

HPLC chromatograms related to deglycosylation of orientin. a blank group; b experimental group at 0 h; c reference compounds of orientin and luteolin in GAM; d experimental group at 24 h. 1: orientin; 2: luteolin

Fig. 3

Fig. 3

Morphological characteristics of strain W12-1. Left: the colony; Right: the cells

Fig. 4

Fig. 4

Neighbor-joining tree based on 16S rDNA gene sequences

Fig. 5

Fig. 5

Time course of deglycosylation of orientin, vitexin and isovitexin (n = 3, mean ± SD)

Fig. 6

Fig. 6

Time course of deglycosylation of orientin in the media with different contents of carbon nutrition (n = 3, mean ± SD)

Fig. 7

Fig. 7

Comparative SDS-PAGE electrophoretogram of strain W 12-1 culture in the media with double content of carbon sources and without carbon sources. M: protein ladder; 1: the culture in the medium without carbon sources; 2: the culture in the medium with double content level of carbon sources

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