The Emergence of Chromosomally Located bla CTX-M-55 in Salmonella From Foodborne Animals in China - PubMed (original) (raw)

The Emergence of Chromosomally Located bla CTX-M-55 in Salmonella From Foodborne Animals in China

Chuan-Zhen Zhang et al. Front Microbiol. 2019.

Abstract

The emergence and increase in prevalence of resistance to cephalosporins amongst isolates of Salmonella from food animals imposes a public health threat. The aim of the present study was to investigate the prevalence and characteristics of CTX-M-producing Salmonella isolates from raw meat and food animals. 27 of 152 (17.76%) Salmonella isolates were ESBL-positive including 21/70 (30%) from food animals and 6/82 (7.32%) from raw meat. CTX-M-55 was the most prevalent ESBL type observed (12/27, 44.44%). 7 of 12 CTX-M-55-positive Salmonella isolates were Salmonella Indiana, 2 were Salmonella Typhimurium, 2 were Salmonella Chester, and the remaining isolate was not typeable. Eight CTX-M-55-positive Salmonella isolates were highly resistant to fluoroquinolones (MICCIP = 64 ug/mL) and co-harbored aac(6')-Ib-cr and oqxAB. Most of the CTX-M-55 positive isolates (11/12) carried bla CTX-M-55 genes on the chromosome, with the remaining isolate carrying this gene on a transferable 280 kb IncHI2 plasmid. A chromosomal bla CTX-M-55 gene from one isolate transferred onto a 250 kb IncHI2 plasmid which was subsequently conjugated into recipient strain J53. PFGE and MLST profiles showed a wide range of strain types were carrying bla CTX-M-55. Our study demonstrates the emergence and prevalence of foodborne Salmonella harboring a chromosomally located bla CTX-M-55 in China. The co-existence of PMQR genes with bla CTX-M-55 in Salmonella isolates suggests co-selection and dissemination of resistance to both fluoroquinolones and cephalosporins in Salmonella via the food chain in China represents a public health concern.

Keywords: Indiana; Salmonella; blaCTX-M-55; chromosomal; transfer.

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Figures

FIGURE 1

FIGURE 1

PFGE fingerprinting patterns and other characteristics of Salmonella isolates harboring _bla_CTX-M-55 genes.

FIGURE 2

FIGURE 2

Southern blot hybridization with the _bla_CTX-M-55 probe (A) and S1-PFGE of S1 nuclease-digested genomic DNA (B). The arrow indicates plasmids in the isolates. OYM4 (lane 1), OYM6 (lane 2), OYM8 (lane 3), OYM9 (lane 4), OYM13 (lane 5), OYZ3 (lane 6), OJM1 (lane 7), HZP3 (lane 8), LWP4 (lane 9), OYM10 (lane 10), PJM1 (lane 11), OYZ4 (lane 12), C-PJM1 (lane 13), C-OYZ4 (lane 14), Marker (H9812).

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