Complete mitochondrial genomes confirm the generic placement of the plateau vole, Neodon fuscus - PubMed (original) (raw)

. 2019 Aug 9;39(8):BSR20182349.

doi: 10.1042/BSR20182349. Print 2019 Aug 30.

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Complete mitochondrial genomes confirm the generic placement of the plateau vole, Neodon fuscus

Jian-Qiu Li et al. Biosci Rep. 2019.

Abstract

The plateau vole, Neodon fuscus is endemic to China and is distributed mainly in Qinghai Province. It is of public health interest, as it is, a potential reservoir of Toxoplasma gondii and the intermediate host of Echinococcus multilocularis However, genetic data of this species are lacking, and its name and taxonomy are still a controversy. In the present study, we determined the nucleotide sequence of the entire mitochondrial (mt) genome of N. fuscus and analyzed its evolutionary relationship. The mitogenome was 16328 bp in length and contained 13 protein-coding genes, 22 genes for transfer RNAs (tRNA), two ribosomal RNA genes and two major noncoding regions (OL region and D-loop region). Most genes were located on the heavy strand. All tRNA genes had typical cloverleaf structures except for tRNASer (GCU) The mt genome of N. fuscus was rich in A+T (58.45%). Maximum likelihood (ML) and Bayesian methods yielded phylogenetic trees from 33 mt genomes of Arvicolinae, in which N. fuscus formed a sister group with Neodon irene and Neodon sikimensis to the exclusion of species of Microtus and other members of the Arvicolinae. Further phylogenetic analyses (ML only) based on the _cyt_b gene sequences also demonstrated that N. fuscus had a close relationship with N. irene The complete mitochondrial genome was successfully assembled and annotated, providing the necessary information for the phylogenetic analyses. Although the name Lasiopodomys fuscus was used in the book 'Wilson & Reeder's Mammal Species of the World', we have confirmed here that its appropriate name is N. fuscus through an analysis of the evolutionary relationships.

Keywords: Arviciolinae; Mitochondrial genome; Neodon fuscus; Phylogenetic analysis; Taxonomy.

© 2019 The Author(s).

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Conflict of interest statement

All animals were handled in strict accordance with good animal practice according to the Animal Ethics Procedures and Guidelines of the People’s Republic of China, and the study was approved by the Animal Ethics Committee of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (No. LVRIAEC2012-007).

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1

Figure 1. The predicted structure of the OL region of Neodon fuscus, Microtus levis, Proedromys liangshanensis and Neodon irene

Figure 2

Figure 2. The phylogenetic relationship of Neodon fuscus with 32 other rodent species inferred from a Bayesian method based on an alignment of complete mt genomes

The numbers at a node represent bootstrap values. ★ Indicates N. fuscus examined in our study. O. collaris and O. curzoniae are used as outgroups.

Figure 3

Figure 3. Phylogenetic trees inferred from _cyt_b gene sequences (from 34 species) using Bayesian method

The number at each node represents the bootstrap value. ★ Indicates N. fuscus examined in our study. Mus musculus is used as outgroup.

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