DNA-binding properties of the Drosophila melanogaster zeste gene product - PubMed (original) (raw)

DNA-binding properties of the Drosophila melanogaster zeste gene product

A Mansukhani et al. Mol Cell Biol. 1988 Feb.

Abstract

The ability of the zeste moiety of beta-galactosidase-zeste fusion proteins synthesized in Escherichia coli to bind specific DNA sequences was examined. Such fusion proteins recognize a region of the white locus upstream of the start of transcription; this region has previously been shown to be required for genetic interaction between the zeste and white loci. Another strong binding site was localized to a region between 50 and 205 nucleotides before the start of the Ubx transcriptional unit; expression of the bithorax complex is also known to be influenced by the zeste locus. Weaker binding sites were also seen in the vicinity of the bxd and Sgs-4 genes, but it is currently unclear whether these binding sites play a role in transvection effects. The DNA-binding activity of the zeste protein is restricted to a domain of approximately 90 amino acids near the N terminus. This domain does not appear to contain homeobox or zinc finger motifs found in other DNA-binding proteins. The DNA-binding domain is not disrupted by any currently characterized zeste mutations.

PubMed Disclaimer

Cited by

The Drosophila zeste locus is nonessential.
Goldberg ML, Colvin RA, Mellin AF. Goldberg ML, et al. Genetics. 1989 Sep;123(1):145-55. doi: 10.1093/genetics/123.1.145. Genetics. 1989. PMID: 2553529 Free PMC article.
A fragment of engrailed regulatory DNA can mediate transvection of the white gene in Drosophila.
Kassis JA, VanSickle EP, Sensabaugh SM. Kassis JA, et al. Genetics. 1991 Aug;128(4):751-61. doi: 10.1093/genetics/128.4.751. Genetics. 1991. PMID: 1655566 Free PMC article.
The Drosophila zeste protein binds cooperatively to sites in many gene regulatory regions: implications for transvection and gene regulation.
Benson M, Pirrotta V. Benson M, et al. EMBO J. 1988 Dec 1;7(12):3907-15. doi: 10.1002/j.1460-2075.1988.tb03277.x. EMBO J. 1988. PMID: 3145199 Free PMC article.
Multimerization of the Drosophila zeste protein is required for efficient DNA binding.
Chen JD, Pirrotta V. Chen JD, et al. EMBO J. 1993 May;12(5):2075-83. doi: 10.1002/j.1460-2075.1993.tb05856.x. EMBO J. 1993. PMID: 8491197 Free PMC article.
An unusual genomic position effect on Drosophila white gene expression: pairing dependence, interactions with zeste, and molecular analysis of revertants.
Hazelrigg T, Petersen S. Hazelrigg T, et al. Genetics. 1992 Jan;130(1):125-38. doi: 10.1093/genetics/130.1.125. Genetics. 1992. PMID: 1732157 Free PMC article.

References

1. Science. 1986 Feb 14;231(4739):699-704 - PubMed
1. DNA. 1985 Apr;4(2):165-70 - PubMed
1. Hereditas. 1974;78(1):97-104 - PubMed
1. Cell. 1986 Sep 12;46(6):885-94 - PubMed
1. Cell. 1985 Aug;42(1):237-47 - PubMed

DNA-binding properties of the Drosophila melanogaster zeste gene product - PubMed (original) (raw)