Randomized Controlled Study to Evaluate Microbial Ecological Effects of CPP-ACP and Cranberry on Dental Plaque - PubMed (original) (raw)

Randomized Controlled Trial

doi: 10.1177/2380084419859871. Epub 2019 Jul 8.

Affiliations

Randomized Controlled Trial

Randomized Controlled Study to Evaluate Microbial Ecological Effects of CPP-ACP and Cranberry on Dental Plaque

N Philip et al. JDR Clin Trans Res. 2020 Apr.

Abstract

Introduction: Ecological approaches to dental caries prevention play a key role in attaining long-term control over the disease and maintaining a symbiotic oral microbiome.

Objectives: This study aimed to investigate the microbial ecological effects of 2 interventional dentifrices: a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) dentifrice and the same dentifrice supplemented with a polyphenol-rich cranberry extract.

Methods: The interventional toothpastes were compared with each other and with an active control fluoride dentifrice in a double-blinded randomized controlled trial. Real-time quantitative polymerase chain reaction (qPCR) analysis was used to determine changes in the bacterial loads of 14 key bacterial species (8 caries associated and 6 health associated) in the dental plaque of trial participants after they used the dentifrices for 5 to 6 wk.

Results: From the baseline to the recall visit, significant differences were observed between the treatment groups in the bacterial loads of 2 caries-associated bacterial species (Streptococcus mutans [P < 0.001] and _Veillonella parvula_ [_P_ < 0.001]) and 3 health-associated bacterial species (_Corynebacterium durum_ [P = 0.008], _Neisseria flavescens_ [_P_ = 0.005], and _Streptococcus sanguinis_ [_P_ < 0.001]). Compared to the fluoride control dentifrice, the CPP-ACP dentifrice demonstrated significant differences for _S. mutans_ (_P_ = 0.032), _C. durum_ (_P_ = 0.007), and _S. sanguinis_ (_P_ < 0.001), while combination CPP-ACP-cranberry dentifrice showed significant differences for _S. mutans_ (_P_ < 0.001), _V. parvula_ (_P_ < 0.001), _N. flavescens_ (_P_ = 0.003), and _S. sanguinis_ (_P_ < 0.001). However, no significant differences were observed in the bacterial load comparisons between the CPP-ACP and combination dentifrices for any of the targeted bacterial species (_P_ > 0.05).

Conclusions: Overall, the results indicate that dentifrices containing CPP-ACP and polyphenol-rich cranberry extracts can influence a species-level shift in the ecology of the oral microbiome, resulting in a microbial community less associated with dental caries (Australian New Zealand Clinical Trial Registry ANZCTR 12618000095268).

Knowledge transfer statement: The results of this randomized controlled trial indicate that dentifrices containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and polyphenol-rich cranberry extracts were able to beneficially modulate the microbial ecology of dental plaque in a group of high caries-risk patients. This could contribute toward lowering the risk of developing new caries lesions, an important goal sought by patients, clinicians, and policy makers.

Keywords: calcium phosphate; caries; dentifrices; dysbiosis; microbial community; proanthocyanidins.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

Supplementary concepts

LinkOut - more resources