Trans-regulation of ecdysterone-induced protein synthesis in Drosophila melanogaster salivary glands - PubMed (original) (raw)
Comparative Study
Trans-regulation of ecdysterone-induced protein synthesis in Drosophila melanogaster salivary glands
E B Dubrovsky et al. Dev Biol. 1988 May.
Abstract
A set of coordinately expressed genes is actively transcribed after a dramatic increase in the ecdysterone titer in late third-instar development of Drosophila melanogaster, as shown by the appearance of a number of puffs in salivary gland chromosomes and by the synthesis of a number of new proteins. Previous work has suggested that a product of the ecs gene, which is located within the 2B3-5 puff, is necessary for providing alterations in transcriptional activity at the sites of ecdysterone-dependent puffs. The experiments reported here were designed to determine whether the ecs gene's regulatory effect on puffing is confirmed by its regulatory effect on the synthesis of ecdysterone-inducible proteins (EIPs). The first series of experiments showed that in salivary glands in vivo ecdysterone induces 24 EIPs and in vitro induces 26 EIPs. The sets of polypeptides revealed are in good conformity. The second set of experiments demonstrated that mutations in the ecs locus disturb EIP synthesis: ecsl(1)t10 and ecsl(1)t143 mutations affect EIP synthesis to a lesser extent, while ecsl(1)t435 and ecsl(1)t324, as well as the 2B3-5 puff deficiency, prevent EIP synthesis completely. The experiments on dosage dependency revealed two EIPs whose rate of synthesis correlates with the dosage of the 2B3-5 X-chromosomal region. These EIPs are shown to be in fact small heat-shock proteins 23 and 28K, which are known to be encoded within the 67B puff and are under dual control--transient and developmental. The final set of experiments followed the 2B3-5 dosage dependency in vitro and showed that 15 EIPs display either an affected rate of synthesis or, mainly, a quicker induction time. Data obtained show that the ecs locus is trans-regulatory and that its product is necessary for spreading the effect of ecdysterone to other loci.
Similar articles
- [The regulation of the ontogenetic expression of the heat-shock genes from puff 67B in the salivary glands of Drosophila melanogaster].
Dubrovskiĭ EB, Kozlova TIu, Rzhetskaia MA. Dubrovskiĭ EB, et al. Ontogenez. 1990 Sep-Oct;21(5):508-16. Ontogenez. 1990. PMID: 2126365 Russian. - Ecdysterone induced protein synthesis in salivary glands and in fat body of Drosophila melanogaster larvae.
Poeting A, Koerwer W, Pongs O. Poeting A, et al. Chromosoma. 1982;87(1):89-102. doi: 10.1007/BF00333511. Chromosoma. 1982. PMID: 6819118 - Steroid regulation of RNAs transcribed from the Drosophila 68c polytene chromosome puff.
Crowley TE, Meyerowitz EM. Crowley TE, et al. Dev Biol. 1984 Mar;102(1):110-21. doi: 10.1016/0012-1606(84)90179-9. Dev Biol. 1984. PMID: 6199239 - In vivo effects of ecdysterone on puff formation, and RNA and protein synthesis in the salivary glands of Rhynchosciara americana.
Alvarenga CA, Winter CE, Stocker AJ, Pueyo MT, Lara FJ. Alvarenga CA, et al. Braz J Med Biol Res. 1991;24(10):985-1002. Braz J Med Biol Res. 1991. PMID: 1724621 Review. - Drosophila cells and ecdysterone: a model system for gene regulation.
Berger EM, Morganelli CM. Berger EM, et al. In Vitro. 1984 Dec;20(12):959-74. doi: 10.1007/BF02619669. In Vitro. 1984. PMID: 6442267 Review.
Cited by
- Mutations in a steroid hormone-regulated gene disrupt the metamorphosis of internal tissues in Drosophila: salivary glands, muscle, and gut.
Restifo LL, White K. Restifo LL, et al. Rouxs Arch Dev Biol. 1992 Jun;201(4):221-234. doi: 10.1007/BF00188753. Rouxs Arch Dev Biol. 1992. PMID: 28305846 - The Broad-Complex gene is a tissue-specific modulator of the ecdysone response of the Drosophila hsp23 gene.
Dubrovsky EB, Dretzen G, Berger EM. Dubrovsky EB, et al. Mol Cell Biol. 1996 Nov;16(11):6542-52. doi: 10.1128/MCB.16.11.6542. Mol Cell Biol. 1996. PMID: 8887683 Free PMC article. - Cytogenetical analysis of the 2B3-4-2B11 region of the X chromosome of Drosophila melanogaster. VII. Influence of the ecs locus on female fertility.
Mazina OM, Belyaeva ES, Zhimulev IF. Mazina OM, et al. Mol Gen Genet. 1991 Jan;225(1):99-105. doi: 10.1007/BF00282647. Mol Gen Genet. 1991. PMID: 1900349 - Chromomeric organization of polytene chromosomes.
Zhimulev IF, Belyaeva ES. Zhimulev IF, et al. Genetica. 1991;85(1):65-72. doi: 10.1007/BF00056107. Genetica. 1991. PMID: 1778476 Review. - The Drosophila Broad-Complex encodes a family of related proteins containing zinc fingers.
DiBello PR, Withers DA, Bayer CA, Fristrom JW, Guild GM. DiBello PR, et al. Genetics. 1991 Oct;129(2):385-97. doi: 10.1093/genetics/129.2.385. Genetics. 1991. PMID: 1743483 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials