Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro - PubMed (original) (raw)
Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro
K Kawakami et al. Proc Natl Acad Sci U S A. 1988 Jul.
Abstract
The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. The purified NF-kappa B has been identified as a 51-kDa polypeptide by UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence. The purified factor activated in vitro transcription of the human immunodeficiency virus type I promoter by binding to an upstream NF-kappa B-binding site.
References
- Science. 1987 Nov 27;238(4831):1247-53 - PubMed
- Proc Natl Acad Sci U S A. 1987 Nov;84(22):7881-5 - PubMed
- EMBO J. 1987 Dec 1;6(12):3761-70 - PubMed
- EMBO J. 1987 Dec 20;6(13):4067-71 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources