The significance of antibodies to poly(adenosine diphosphate-ribose) in systemic lupus erythematosus - PubMed (original) (raw)

The significance of antibodies to poly(adenosine diphosphate-ribose) in systemic lupus erythematosus

E E Okolie et al. Clin Exp Immunol. 1979 Apr.

Abstract

Poly(adenosine diphosphate-ribose) and ds-DNA binding activity have been measured in thirty-nine systemic lupus erythematosus (SLE) sera, nineteen rheumatoid arthritis sera, fourteen sera from non-SLE rheumatic and non-rheumatic diseases and in ten normal sera. Antibodies to poly(ADP-ribose) were found only in the SLE and in three SLE-like rheumatic diseases. Anti-DNA antibodies, on the other hand, were found not only in the SLE and SLE-like diseases, but also in rheumatoid arthritis and chronic active hepatitis. Estimation of poly(ADP-ribose) binding was, therefore, more specific for, and more discriminatory of SLE from other diseases, than the estimation of ds-DNA binding. The results indicate that the estimation of poly(ADP-ribose) binding in serum may be more useful in the diagnosis of SLE than the presently employed estimation of DNA binding using the Amersham kit. DNA-anti-DNA immune complexes are detected in some of the SLE sera after deoxyribonuclease I digestion, confirming earlier reports of the existence of circulating DNA-anti-DNA complexes in SLE patients. Snake venom phosphodiesterase treatment of some of the SLE sera also resulted in increased poly(ADP-ribose) binding activity, suggesting the existence of poly(ADP-ribose)-anti-poly(ADP-ribose) immune complexes in the circulation of SLE patients. This observation raises the possiblity that poly(ADP-ribose) immune complexes may play some part in the pathogenesis of some cases of SLE.

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References

    1. J Clin Invest. 1959 Nov;38:1996-2016 - PubMed
    1. J Immunol. 1961 Oct;87:477-84 - PubMed
    1. J Infect Dis. 1958 Nov-Dec;103(3):239-62 - PubMed
    1. Annu Rev Biochem. 1977;46:95-116 - PubMed
    1. Rev Physiol Biochem Pharmacol. 1976;76:1-58, 177 - PubMed

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