Instantaneous aortic blood flow measurement with range-gated Doppler flowmeter in anesthetized rat - PubMed (original) (raw)
Instantaneous aortic blood flow measurement with range-gated Doppler flowmeter in anesthetized rat
J Benessiano et al. J Pharmacol Methods. 1985 Sep.
Abstract
The availability of a range-gated Doppler flowmeter system enabled us to construct miniaturized probes using piezoelectric crystals that emit an 8 MHz signal and receive the reflected sound waves from passing blood cells. The finished flow probe are 4 mm long and 3 mm in external diameter with lumen diameter appropriate to be placed around the ascending aorta in the rat. The accuracy of the Doppler method in measuring cardiac output in the rat was established by the demonstration of a significant correlation between cardiac output simultaneously measured from ultrasonic (Qd) and thermodilution (Qt) procedures in anesthetized Wistar rats (Qd = 0.55, Qt - 6.67 cm3/min, r = 0.69, p less than 0.001). An average Qd determination is 52.5% +/- 16% of average Qt. From aortic blood pressure and phasic aortic blood velocity, we have compared hemodynamic and cardiovascular functions in 15 spontaneously hypertensive rats and in 15 control Wistar-Kyoto rats under pentobarbital sodium anesthesia. The present study demonstrates that phasic aortic blood flow can be quantified easily and accurately in anesthetized rats by using a range-gated Doppler flowmeter and an implantable perivascular flow probe, and should provide a relatively simply method for investigating hemodynamic characteristics in models of disease such as ventricular hypertrophy and hypertension.
Similar articles
- Regional blood flow measurement with pulsed Doppler flowmeter in conscious rat.
Haywood JR, Shaffer RA, Fastenow C, Fink GD, Brody MJ. Haywood JR, et al. Am J Physiol. 1981 Aug;241(2):H273-8. doi: 10.1152/ajpheart.1981.241.2.H273. Am J Physiol. 1981. PMID: 6455924 - Hemodynamic and biochemical characteristics of the aorta in the WKY, SHR, WKHT, and WKHA rat strains.
Ricci MA, Slaiby JM, Hendley ED, Stirewalt W, Cloutier L, Nichols P, Evans JN. Ricci MA, et al. Ann N Y Acad Sci. 1996 Nov 18;800:121-30. doi: 10.1111/j.1749-6632.1996.tb33303.x. Ann N Y Acad Sci. 1996. PMID: 8958987 - Measurement of cardiac output by the thermodilution method in conscious spontaneously hypertensive rats.
Watanabe K, Nishio T, Mori C, Kihara M, Yamori Y. Watanabe K, et al. Jpn Heart J. 1984 Nov;25(6):1051-8. doi: 10.1536/ihj.25.1051. Jpn Heart J. 1984. PMID: 6241634 - Effect of chronic dihydropyridine (isradipine) on the large arterial walls of spontaneously hypertensive rats.
Levy BI, Duriez M, Phillipe M, Poitevin P, Michel JB. Levy BI, et al. Circulation. 1994 Dec;90(6):3024-33. doi: 10.1161/01.cir.90.6.3024. Circulation. 1994. PMID: 7994851 - The effects of voluntary running on cardiac mass and aortic compliance in Wistar-Kyoto and spontaneously hypertensive rats.
Kingwell BA, Arnold PJ, Jennings GL, Dart AM. Kingwell BA, et al. J Hypertens. 1998 Feb;16(2):181-5. doi: 10.1097/00004872-199816020-00008. J Hypertens. 1998. PMID: 9535145
Cited by
- Monitoring of Cardiorespiratory Parameters in Rats-Validation Based on Pharmacological Stimulation.
Miklosz J, Kalaska B, Zajaczkowski S, Pawlak D, Mogielnicki A. Miklosz J, et al. Pharmaceuticals (Basel). 2021 Nov 25;14(12):1223. doi: 10.3390/ph14121223. Pharmaceuticals (Basel). 2021. PMID: 34959624 Free PMC article. - Polymeric perfluorocarbon nanoemulsions are ultrasound-activated wireless drug infusion catheters.
Zhong Q, Yoon BC, Aryal M, Wang JB, Ilovitsh T, Baikoghli MA, Hosseini-Nassab N, Karthik A, Cheng RH, Ferrara KW, Airan RD. Zhong Q, et al. Biomaterials. 2019 Jun;206:73-86. doi: 10.1016/j.biomaterials.2019.03.021. Epub 2019 Mar 20. Biomaterials. 2019. PMID: 30953907 Free PMC article. - Breakers of advanced glycation end products restore large artery properties in experimental diabetes.
Wolffenbuttel BH, Boulanger CM, Crijns FR, Huijberts MS, Poitevin P, Swennen GN, Vasan S, Egan JJ, Ulrich P, Cerami A, Lévy BI. Wolffenbuttel BH, et al. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4630-4. doi: 10.1073/pnas.95.8.4630. Proc Natl Acad Sci U S A. 1998. PMID: 9539789 Free PMC article. - Aminoguanidine prevents age-related arterial stiffening and cardiac hypertrophy.
Corman B, Duriez M, Poitevin P, Heudes D, Bruneval P, Tedgui A, Levy BI. Corman B, et al. Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1301-6. doi: 10.1073/pnas.95.3.1301. Proc Natl Acad Sci U S A. 1998. PMID: 9448326 Free PMC article. - Chronic blockade of AT2-subtype receptors prevents the effect of angiotensin II on the rat vascular structure.
Levy BI, Benessiano J, Henrion D, Caputo L, Heymes C, Duriez M, Poitevin P, Samuel JL. Levy BI, et al. J Clin Invest. 1996 Jul 15;98(2):418-25. doi: 10.1172/JCI118807. J Clin Invest. 1996. PMID: 8755652 Free PMC article.