Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cells - PubMed (original) (raw)
Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cells
U Siebenlist et al. Mol Cell Biol. 1988 Feb.
Abstract
We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which distinct segments of the c-myc gene were used as probes, an increased blocking to elongation of nascent c-myc transcripts was shown during the early phase of differentiation. During a later phase, however, a loss of transcriptional initiation was observed. This loss of promoter activity correlated well with dramatic changes in the chromatin structure of the c-myc gene, as determined by DNase I-hypersensitive site analysis. In particular, two hypersensitive sites near the two major c-myc promoters disappeared at the time that promotion abated. The newly described, later-acting negative transcriptional control of c-myc also correlated temporally with the inability to reverse the downregulation of the c-myc message quickly on withdrawal of the differentiating agent. Therefore, a terminal step during differentiation may be linked to the later-acting mode of transcriptional regulation of c-myc. The evidence presented in this report has implications for tumorigenesis in Burkitt lymphomas, in which the germ line, nontranslocated c-myc allele is transcriptionally silent.
Similar articles
- Chromatin structure of transcriptionally active and inactive human c-myc alleles.
Dyson PJ, Littlewood TD, Forster A, Rabbitts TH. Dyson PJ, et al. EMBO J. 1985 Nov;4(11):2885-91. doi: 10.1002/j.1460-2075.1985.tb04018.x. EMBO J. 1985. PMID: 2998760 Free PMC article. - c-myc gene inactivation during induced maturation of HL-60 cells. Transcriptional repression and loss of a specific DNAse I hypersensitive site.
High KA, Stolle CA, Schneider JW, Hu W, Benz EJ Jr. High KA, et al. J Clin Invest. 1987 Jan;79(1):93-9. doi: 10.1172/JCI112814. J Clin Invest. 1987. PMID: 3540012 Free PMC article. - Hold back of RNA polymerase II at the transcription start site mediates down-regulation of c-myc in vivo.
Strobl LJ, Eick D. Strobl LJ, et al. EMBO J. 1992 Sep;11(9):3307-14. doi: 10.1002/j.1460-2075.1992.tb05409.x. EMBO J. 1992. PMID: 1505520 Free PMC article. - The regulatory strategies of c-myc and c-fos proto-oncogenes share some common mechanisms.
Blanchard JM, Piechaczyk M, Fort P, Bonnieu A, Mechti N, Rech J, Cuny M, Lebleu B, Jeanteur P. Blanchard JM, et al. Biochimie. 1988 Jul;70(7):877-84. doi: 10.1016/0300-9084(88)90228-3. Biochimie. 1988. PMID: 3145022 Review. - The HL60 cell line: a model system for studying human myeloid cell differentiation.
Birnie GD. Birnie GD. Br J Cancer Suppl. 1988 Dec;9:41-5. Br J Cancer Suppl. 1988. PMID: 3076064 Free PMC article. Review.
Cited by
- A map of gene expression in neutrophil-like cell lines.
Rincón E, Rocha-Gregg BL, Collins SR. Rincón E, et al. BMC Genomics. 2018 Aug 1;19(1):573. doi: 10.1186/s12864-018-4957-6. BMC Genomics. 2018. PMID: 30068296 Free PMC article. - Induction of Myc-intron-binding polypeptides MIBP1 and RFX1 during retinoic acid-mediated differentiation of haemopoietic cells.
Zajac-Kaye M, Ben-Baruch N, Kastanos E, Kaye FJ, Allegra C. Zajac-Kaye M, et al. Biochem J. 2000 Feb 1;345 Pt 3(Pt 3):535-41. Biochem J. 2000. PMID: 10642512 Free PMC article. - The immunoglobulin heavy chain locus control region increases histone acetylation along linked c-myc genes.
Madisen L, Krumm A, Hebbes TR, Groudine M. Madisen L, et al. Mol Cell Biol. 1998 Nov;18(11):6281-92. doi: 10.1128/MCB.18.11.6281. Mol Cell Biol. 1998. PMID: 9774645 Free PMC article. - Nucleosomal structures of c-myc promoters with transcriptionally engaged RNA polymerase II.
Albert T, Mautner J, Funk JO, Hörtnagel K, Pullner A, Eick D. Albert T, et al. Mol Cell Biol. 1997 Aug;17(8):4363-71. doi: 10.1128/MCB.17.8.4363. Mol Cell Biol. 1997. PMID: 9234694 Free PMC article. - Identification of sequences in c-myc mRNA that regulate its steady-state levels.
Yeilding NM, Rehman MT, Lee WM. Yeilding NM, et al. Mol Cell Biol. 1996 Jul;16(7):3511-22. doi: 10.1128/MCB.16.7.3511. Mol Cell Biol. 1996. PMID: 8668167 Free PMC article.
References
- Biochemistry. 1977 Oct 18;16(21):4743-51 - PubMed
- Exp Cell Res. 1985 Jan;156(1):198-212 - PubMed
- Science. 1979 May 25;204(4395):868-70 - PubMed
- Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
- Proc Natl Acad Sci U S A. 1980 May;77(5):2936-40 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources