The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity - PubMed (original) (raw)
The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity
R Vlasak et al. J Virol. 1988 Dec.
Abstract
In addition to members of the Orthomyxoviridae and Paramyxoviridae, several coronaviruses have been shown to possess receptor-destroying activities. Purified bovine coronavirus (BCV) preparations have an esterase activity which inactivates O-acetylsialic acid-containing receptors on erythrocytes. Diisopropyl fluorophosphate (DFP) completely inhibits this receptor-destroying activity of BCV, suggesting that the viral enzyme is a serine esterase. Treatment of purified BCV with [3H]DFP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins revealed that the E3 protein was specifically phosphorylated. This finding suggests that the esterase/receptor-destroying activity of BCV is associated with the E3 protein. Furthermore, treatment of BCV with DFP dramatically reduced its infectivity in a plaque assay. It is assumed that the esterase activity of BCV is required in an early step of virus replication, possibly during virus entry or uncoating.
Similar articles
- Comparison of hemagglutinating, receptor-destroying, and acetylesterase activities of avirulent and virulent bovine coronavirus strains.
Storz J, Zhang XM, Rott R. Storz J, et al. Arch Virol. 1992;125(1-4):193-204. doi: 10.1007/BF01309637. Arch Virol. 1992. PMID: 1642550 Free PMC article. - Isolated HE-protein from hemagglutinating encephalomyelitis virus and bovine coronavirus has receptor-destroying and receptor-binding activity.
Schultze B, Wahn K, Klenk HD, Herrler G. Schultze B, et al. Virology. 1991 Jan;180(1):221-8. doi: 10.1016/0042-6822(91)90026-8. Virology. 1991. PMID: 1984649 Free PMC article. - Characterization of the receptor-destroying enzyme activity from infectious salmon anaemia virus.
Kristiansen M, Frøystad MK, Rishovd AL, Gjøen T. Kristiansen M, et al. J Gen Virol. 2002 Nov;83(Pt 11):2693-2697. doi: 10.1099/0022-1317-83-11-2693. J Gen Virol. 2002. PMID: 12388804 - 9-O-acetylated sialic acid, a receptor determinant for influenza C virus and coronaviruses.
Herrler G, Szepanski S, Schultze B. Herrler G, et al. Behring Inst Mitt. 1991 Jul;(89):177-84. Behring Inst Mitt. 1991. PMID: 1930096 Review. - Infectious mechanisms of enteropathogenic bovine coronaviruses.
Storz J, Zhang XM. Storz J, et al. Behring Inst Mitt. 1991 Jul;(89):130-7. Behring Inst Mitt. 1991. PMID: 1656930 Review.
Cited by
- Diversifying selection identified in immune epitopes of bovine coronavirus isolates from Irish cattle.
Russell T, Lozano JM, Challinor C, Barry G. Russell T, et al. J Gen Virol. 2024 Aug;105(8):002019. doi: 10.1099/jgv.0.002019. J Gen Virol. 2024. PMID: 39158563 - Human Coronavirus OC43 as a Low-Risk Model to Study COVID-19.
Kim MI, Lee C. Kim MI, et al. Viruses. 2023 Feb 20;15(2):578. doi: 10.3390/v15020578. Viruses. 2023. PMID: 36851792 Free PMC article. Review. - Destruction of the vascular viral receptor in infectious salmon anaemia provides in vivo evidence of homologous attachment interference.
Aamelfot M, Fosse JH, Viljugrein H, Ploss FB, Benestad SL, McBeath A, Christiansen DH, Garver K, Falk K. Aamelfot M, et al. PLoS Pathog. 2022 Oct 14;18(10):e1010905. doi: 10.1371/journal.ppat.1010905. eCollection 2022 Oct. PLoS Pathog. 2022. PMID: 36240255 Free PMC article. - Reovirus Efficiently Reassorts Genome Segments during Coinfection and Superinfection.
Thoner TW Jr, Meloy MM, Long JM, Diller JR, Slaughter JC, Ogden KM. Thoner TW Jr, et al. J Virol. 2022 Sep 28;96(18):e0091022. doi: 10.1128/jvi.00910-22. Epub 2022 Sep 12. J Virol. 2022. PMID: 36094315 Free PMC article. - Viral and cellular translation during SARS-CoV-2 infection.
Eriani G, Martin F. Eriani G, et al. FEBS Open Bio. 2022 Sep;12(9):1584-1601. doi: 10.1002/2211-5463.13413. Epub 2022 Apr 25. FEBS Open Bio. 2022. PMID: 35429230 Free PMC article. Review.
References
- J Virol. 1973 Dec;12(6):1457-65 - PubMed
- Virology. 1972 Dec;50(3):640-52 - PubMed
- Virology. 1974 Jan;57(1):227-37 - PubMed
- Virology. 1974 Oct;61(2):397-410 - PubMed
- Virology. 1974 Nov;62(1):125-33 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources