Asynchronous DNA replication within the human beta-globin gene locus - PubMed (original) (raw)

Asynchronous DNA replication within the human beta-globin gene locus

E Epner et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

The timing of DNA replication of the human beta-globin gene locus has been studied by blot hybridization of newly synthesized BrdUrd-substituted DNA from cells in different stages of the S phase. Using probes that span greater than 120 kilobases across the human beta-globin gene locus, we show that the majority of this domain replicates in early S phase in the human erythroleukemia cell line K562 and in middle-to-late S phase in the lymphoid cell line Manca. However, in K562 cells three small regions display a strikingly different replication pattern than adjacent sequences. These islands, located in the inter-gamma-globin gene region and approximately 20 kilobases 5' to the epsilon-globin gene and 20 kilobases 3' to the beta-globin gene, replicate later and throughout S phase. A similar area is also present in the alpha-globin gene region in K562 cells. We suggest that these regions may represent sites of termination of replication forks.

PubMed Disclaimer

Similar articles

• Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.
  Cimbora DM, Schübeler D, Reik A, Hamilton J, Francastel C, Epner EM, Groudine M. Cimbora DM, et al. Mol Cell Biol. 2000 Aug;20(15):5581-91. doi: 10.1128/MCB.20.15.5581-5591.2000. Mol Cell Biol. 2000. PMID: 10891496 Free PMC article.
• alpha-Globulin sequences are located in a region of early-replicating DNA in murine erythroleukemia cells.
  Furst A, Brown EH, Braunstein JD, Schildkraut CL. Furst A, et al. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1023-7. doi: 10.1073/pnas.78.2.1023. Proc Natl Acad Sci U S A. 1981. PMID: 6262751 Free PMC article.
• The mammalian beta globin origin of DNA replication.
  Aladjem MI. Aladjem MI. Front Biosci. 2004 Sep 1;9:2540-7. doi: 10.2741/1415. Front Biosci. 2004. PMID: 15358579 Review.
• DNase I sensitive site in the core region of the human beta-globin origin of replication.
  Djeliova V, Russev G, Anachkova B. Djeliova V, et al. J Cell Biochem. 2002;87(3):279-83. doi: 10.1002/jcb.10298. J Cell Biochem. 2002. PMID: 12397609
• Use of long sequence alignments to study the evolution and regulation of mammalian globin gene clusters.
  Hardison R, Miller W. Hardison R, et al. Mol Biol Evol. 1993 Jan;10(1):73-102. doi: 10.1093/oxfordjournals.molbev.a039991. Mol Biol Evol. 1993. PMID: 8383794 Review.

Cited by

• A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells.
  Zhang Y, Huang L, Fu H, Smith OK, Lin CM, Utani K, Rao M, Reinhold WC, Redon CE, Ryan M, Kim R, You Y, Hanna H, Boisclair Y, Long Q, Aladjem MI. Zhang Y, et al. Nat Commun. 2016 Jun 8;7:11748. doi: 10.1038/ncomms11748. Nat Commun. 2016. PMID: 27272143 Free PMC article.
• Allele-specific analysis of DNA replication origins in mammalian cells.
  Bartholdy B, Mukhopadhyay R, Lajugie J, Aladjem MI, Bouhassira EE. Bartholdy B, et al. Nat Commun. 2015 May 19;6:7051. doi: 10.1038/ncomms8051. Nat Commun. 2015. PMID: 25987481 Free PMC article.
• USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator.
  Hassan-Zadeh V, Chilaka S, Cadoret JC, Ma MK, Boggetto N, West AG, Prioleau MN. Hassan-Zadeh V, et al. PLoS Biol. 2012;10(3):e1001277. doi: 10.1371/journal.pbio.1001277. Epub 2012 Mar 6. PLoS Biol. 2012. PMID: 22412349 Free PMC article.
• Defining the replication program through the chromatin landscape.
  Ding Q, MacAlpine DM. Ding Q, et al. Crit Rev Biochem Mol Biol. 2011 Apr;46(2):165-79. doi: 10.3109/10409238.2011.560139. Crit Rev Biochem Mol Biol. 2011. PMID: 21417598 Free PMC article. Review.
• Interactions of MCP1 with components of the replication machinery in mammalian cells.
  Bronze-da-Rocha E, Lin CM, Shimura T, Aladjem MI. Bronze-da-Rocha E, et al. Int J Biol Sci. 2011 Feb 17;7(2):193-208. doi: 10.7150/ijbs.7.193. Int J Biol Sci. 2011. PMID: 21383955 Free PMC article.

References

1. 1. J Cell Physiol Suppl. 1982;1:137-43 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1981 May;78(5):3058-62 - PubMed
3. 1. Cell. 1982 Aug;30(1):131-9 - PubMed
4. 1. Cell. 1982 Nov;31(1):121-9 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2127-31 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database