Characterization and immunogenicity of bone marrow-derived mesenchymal stem cells under osteoporotic conditions - PubMed (original) (raw)
. 2020 Mar;63(3):429-442.
doi: 10.1007/s11427-019-1555-9. Epub 2019 Dec 20.
Affiliations
- PMID: 31879847
- DOI: 10.1007/s11427-019-1555-9
Characterization and immunogenicity of bone marrow-derived mesenchymal stem cells under osteoporotic conditions
Yingkang Huang et al. Sci China Life Sci. 2020 Mar.
Abstract
Mesenchymal stem cells (MSCs) are characterized by their multilineage potential and low immunogenicity. However, the properties of MSCs under pathological conditions are unclear. The current study investigated the differentiation potential and immunological characteristics of bone marrow-derived MSCs from ovariectomized-osteoporotic rats (OP-BMSCs). Although the expression of cell morphology- and stemness-related surface markers was similar between OP-BMSCs and BMSCs from healthy rats (H-BMSCs), the proliferation rate was significantly decreased compared with that of H-BMSCs. Regarding multilineage potential, osteogenesis and chondrogenesis abilities of OP-BMSCs decreased, but the adipogenesis ability was significantly enhanced compared with that of H-BMSCs. As expected, decreased osteogenesis following osteogenic induction resulted in reduced expression of β-catenin, osteocalcin, and runt-related transcription factor 2 in OP-BMSCs. Remarkably, the expression of the co-stimulatory proteins CD40 and CD80 was significantly higher, whereas the expression of the negative co-stimulatory molecule programmed cell death ligand 1 was significantly lower in the OP-BMSCs than that in H-BMSCs. Consequently, H-BMSCs inhibited the proliferation and secretion of inflammatory cytokines from anti-CD3 antibody-activated T cells, whereas OP-BMSCs did not. These results indicate that decreased osteogenesis and increased immunogenicity of OP-BMSCs contribute to bone loss in osteoporosis.
Keywords: adipogenesis; chondrogenesis; immunogenicity; mesenchymal stem cells; osteogenesis; osteoporosis.
Similar articles
- Extracellular vesicles from GPNMB-modified bone marrow mesenchymal stem cells attenuate bone loss in an ovariectomized rat model.
Huang B, Su Y, Shen E, Song M, Liu D, Qi H. Huang B, et al. Life Sci. 2021 May 1;272:119208. doi: 10.1016/j.lfs.2021.119208. Epub 2021 Feb 11. Life Sci. 2021. PMID: 33582177 - Tissue source determines the differentiation potentials of mesenchymal stem cells: a comparative study of human mesenchymal stem cells from bone marrow and adipose tissue.
Xu L, Liu Y, Sun Y, Wang B, Xiong Y, Lin W, Wei Q, Wang H, He W, Wang B, Li G. Xu L, et al. Stem Cell Res Ther. 2017 Dec 6;8(1):275. doi: 10.1186/s13287-017-0716-x. Stem Cell Res Ther. 2017. PMID: 29208029 Free PMC article. - Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model.
Chen M, Han H, Zhou S, Wen Y, Chen L. Chen M, et al. Stem Cell Res Ther. 2021 Mar 12;12(1):173. doi: 10.1186/s13287-021-02239-3. Stem Cell Res Ther. 2021. PMID: 33712069 Free PMC article. - Reciprocal Effect of Environmental Stimuli to Regulate the Adipogenesis and Osteogenesis Fate Decision in Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs).
Xu X, Zhao L, Terry PD, Chen J. Xu X, et al. Cells. 2023 May 16;12(10):1400. doi: 10.3390/cells12101400. Cells. 2023. PMID: 37408234 Free PMC article. Review. - Osteogenesis of bone marrow mesenchymal stem cell in hyperglycemia.
Luo M, Zhao Z, Yi J. Luo M, et al. Front Endocrinol (Lausanne). 2023 Jun 21;14:1150068. doi: 10.3389/fendo.2023.1150068. eCollection 2023. Front Endocrinol (Lausanne). 2023. PMID: 37415664 Free PMC article. Review.
Cited by
- diABZI and poly(I:C) inhibit osteoclastic bone resorption by inducing IRF7 and IFIT3.
Huang Y, Zhang M, Zhang J, Liu S, Li D, Qiao Z, Yao H, Shi Q, Zhou X, Ma F. Huang Y, et al. J Bone Miner Res. 2024 Aug 21;39(8):1132-1146. doi: 10.1093/jbmr/zjae093. J Bone Miner Res. 2024. PMID: 38874138 Free PMC article. - BushenHuoxue formula promotes osteogenic differentiation via affecting Hedgehog signaling pathway in bone marrow stem cells to improve osteoporosis symptoms.
Chen Y, Wei Z, Shi H, Wen X, Wang Y, Wei R. Chen Y, et al. PLoS One. 2023 Nov 29;18(11):e0289912. doi: 10.1371/journal.pone.0289912. eCollection 2023. PLoS One. 2023. PMID: 38019761 Free PMC article. - Cellular senescence in skeletal disease: mechanisms and treatment.
He X, Hu W, Zhang Y, Chen M, Ding Y, Yang H, He F, Gu Q, Shi Q. He X, et al. Cell Mol Biol Lett. 2023 Oct 27;28(1):88. doi: 10.1186/s11658-023-00501-5. Cell Mol Biol Lett. 2023. PMID: 37891477 Free PMC article. Review. - Screening diagnostic markers of osteoporosis based on ferroptosis of osteoblast and osteoclast.
Cao Z, Xue Y, Wang J. Cao Z, et al. Aging (Albany NY). 2023 Sep 28;15(18):9391-9407. doi: 10.18632/aging.204945. Epub 2023 Sep 28. Aging (Albany NY). 2023. PMID: 37770229 Free PMC article. - Cell Sheet Technology: An Emerging Approach for Tendon and Ligament Tissue Engineering.
Li Y, Deng T, Aili D, Chen Y, Zhu W, Liu Q. Li Y, et al. Ann Biomed Eng. 2024 Feb;52(2):141-152. doi: 10.1007/s10439-023-03370-3. Epub 2023 Sep 20. Ann Biomed Eng. 2024. PMID: 37731091 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous