Conditional enhancement of liver-specific gene transcription - PubMed (original) (raw)

Conditional enhancement of liver-specific gene transcription

K S Zaret et al. Proc Natl Acad Sci U S A. 1988 Dec.

Abstract

We sought to develop a cell line in which liver-specific transcription could be induced at will, to facilitate the study of factors that cause hepatocyte-specific transcription of the serum albumin gene in mice. We therefore created the H2.35 cell line from mouse hepatocytes infected with a temperature-sensitive strain of simian virus 40. During routine propagation at the permissive temperature, H2.35 cells exhibit extremely low levels of albumin transcription and mRNA. Albumin mRNA increases at least 100-fold when H2.35 cells are cultured at the restrictive temperature and in serum-free medium on a collagen substratum; the two latter conditions maintain the differentiated state of primary hepatocyte cultures. Although a major cause of the mRNA increase is posttranscriptional, the transcription rates of albumin and other liver-specific genes increase significantly. Transient-transfection experiments demonstrated that an induction of transcription is caused by activation of an albumin upstream sequence that was previously shown to enhance liver-specific transcription in transgenic mice. Thus, hepatocyte differentiation appears to be maintained in part by extracellular signals that stimulate the activity of a tissue-specific enhancer element.

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References

    1. Cell. 1979 Apr;16(4):777-85 -PubMed
    1. Mol Cell Biol. 1985 Oct;5(10):2633-41 -PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 -PubMed
    1. Cell. 1980 May;20(1):95-105 -PubMed
    1. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 -PubMed

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