Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins - PubMed (original) (raw)

. 2020 Apr 3;19(4):1800-1811.

doi: 10.1021/acs.jproteome.0c00048. Epub 2020 Mar 24.

Samira R Aili 2, Nathan Téné 1, Valentine Barassé 1, Christophe Klopp 3, Alain Dejean 4 5, R Manjunatha Kini 6 7, Mrinalini 6, Laurent Coquet 8, Thierry Jouenne 8, Benjamin Lefranc 9, Jérôme Leprince 9, Pierre Escoubas 10, Graham M Nicholson 2, Michel Treilhou 1, Elsa Bonnafé 1

Affiliations

• PMID: 32182430
• DOI: 10.1021/acs.jproteome.0c00048

Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins

Axel Touchard et al. J Proteome Res. 2020.

Abstract

Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by _O-_fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.

Keywords: glycosylated toxin; peptidome; polycationic α-helix; predation; pyroglutamate; reversible neurotoxicity.

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