Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women - PubMed (original) (raw)

Potato Protein Isolate Stimulates Muscle Protein Synthesis at Rest and with Resistance Exercise in Young Women

Sara Y Oikawa et al. Nutrients. 2020.

Abstract

Skeletal muscle myofibrillar protein synthesis (MPS) increases in response to protein feeding and to resistance exercise (RE), where each stimuli acts synergistically when combined. The efficacy of plant proteins such as potato protein (PP) isolate to stimulate MPS is unknown. We aimed to determine the effects of PP ingestion on daily MPS with and without RE in healthy women. In a single blind, parallel-group design, 24 young women (21 ± 3 years, n = 12/group) consumed a weight-maintaining baseline diet containing 0.8 g/kg/d of protein before being randomized to consume either 25 g of PP twice daily (1.6 g/kg/d total protein) or a control diet (CON) (0.8 g/kg/d total protein) for 2 wks. Unilateral RE (~30% of maximal strength to failure) was performed thrice weekly with the opposite limb serving as a non-exercised control (Rest). MPS was measured by deuterated water ingestion at baseline, following supplementation (Rest), and following supplementation + RE (Exercise). Ingestion of PP stimulated MPS by 0.14 ± 0.09 %/d at Rest, and by 0.32 ± 0.14 %/d in the Exercise limb. MPS was significantly elevated by 0.20 ± 0.11 %/d in the Exercise limb in CON (P = 0.008). Consuming PP to increase protein intake to levels twice the recommended dietary allowance for protein augmented rates of MPS. Performance of RE stimulated MPS regardless of protein intake. PP is a high-quality, plant-based protein supplement that augments MPS at rest and following RE in healthy young women.

Keywords: muscle protein synthesis; potato protein; resistance exercise.

PubMed Disclaimer

Conflict of interest statement

S.Y.O, R.B., T.M.H., J.C.M., C.L., C.M., and S.K.B. declare no conflicts of interest. S.M.P. reports having received honoraria, travel expenses, and/or competitive grant support from the following: Dairy Farmers of Canada, US National Dairy Council, Egg Farmers of Canada, and the US National Dairy Export Council. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1

Figure 1

Study schematic. PP, potato protein. * indicates a unilateral resistance exercise session. ↑ indicates one muscle biopsy sample.

Figure 2

Figure 2

Integrated myofibrillar protein synthesis (%/day) at Baseline, and at Rest and with Exercise during the supplementation period. The horizontal line represents the mean, and the whiskers represent the 95% confidence interval. Means that do not share a letter are significantly different within the same group, p = 0.008. FSR, fractional synthetic rate; PP, potato protein supplemented group; and CON, control diet group.

Figure 3

Figure 3

Total protein expression at Baseline (Day 0), and at Rest and Exercise during the supplementation period at Day 14. (A) Total protein expression of Akt; (B) total protein expression of mTOR, and (C) total protein expression of s6. The horizontal line represents the mean, and the whiskers represent the 95% confidence interval. * Indicates significantly greater than Baseline, p < 0.0001. PP, potato protein supplemented group; CON, control diet group.

Similar articles

Cited by

References

    1. Hector A.J., McGlory C., Phillips S.M. The influence of mechanical loading on skeletal muscle protein turnover. Cell. Mol. Exerc. Physiol. 2015;4:e8. doi: 10.7457/cmep.v4i1.e8. - DOI
    1. Burd N.A., West D.W., Staples A.W., Atherton P.J., Baker J.M., Moore D.R., Holwerda A.M., Parise G., Rennie M.J., Baker S.K., et al. Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men. PLoS ONE. 2010;5:e12033. doi: 10.1371/journal.pone.0012033. - DOI - PMC - PubMed
    1. Mitchell C.J., Churchward-Venne T.A., West D.W., Burd N.A., Breen L., Baker S.K., Phillips S.M. Resistance exercise load does not determine training-mediated hypertrophic gains in young men. J. Appl. Physiol. 2012;113:71–77. doi: 10.1152/japplphysiol.00307.2012. - DOI - PMC - PubMed
    1. Morton R.W., Oikawa S.Y., Wavell C.G., Mazara N., McGlory C., Quadrilatero J., Baechler B.L., Baker S.K., Phillips S.M. Neither load nor systemic hormones determine resistance training-mediated hypertrophy or strength gains in resistance-trained young men. J. Appl. Physiol. 2016;121:129–138. doi: 10.1152/japplphysiol.00154.2016. - DOI - PMC - PubMed
    1. Stokes T., Hector A.J., Morton R.W., McGlory C., Phillips S.M. Recent Perspectives Regarding the Role of Dietary Protein for the Promotion of Muscle Hypertrophy with Resistance Exercise Training. Nutrients. 2018;10:180. doi: 10.3390/nu10020180. - DOI - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources