Molecular cloning of a cDNA encoding the human Sm-D autoantigen - PubMed (original) (raw)

Comparative Study

Molecular cloning of a cDNA encoding the human Sm-D autoantigen

L A Rokeach et al. Proc Natl Acad Sci U S A. 1988 Jul.

Abstract

Antibodies to the Sm-D polypeptide antigen are closely associated with the rheumatic disease systemic lupus erythematosus. Sm-D exists in the cell as one of the core proteins of the small nuclear ribonucleoprotein complexes implicated in RNA processing. We have isolated a cDNA clone, D45-2, coding for the Sm-D human nuclear antigen by screening a human B-lymphocyte cDNA library with synthetic oligonucleotide probes. The 1633-base-pair clone contains an open reading frame (ORF) 357 nucleotides long, capable of encoding a 13,282-dalton polypeptide. The Sm-D coding region is initiated at an AUG codon downstream from a sequence with excellent match to the consensus for the eukaryotic ribosome-binding site. The Sm-D ORF is preceded by a 150-nucleotide-long untranslated leader and followed by a 1126-nucleotide-long untranslated region containing four putative poly(A) signals. The predicted amino acid sequence reveals a (Gly-Arg)9 repeated motif at the C terminus, which may constitute one of the Sm-D immunoreactive determinants. Moreover, this C terminus shows interesting features: (i) a good homology to protamines as expected for a nucleic acid binding protein and (ii) a striking similarity to a region in the Epstein-Barr nuclear antigen.

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References

1. 1. J Cell Biol. 1987 Apr;104(4):817-29 - PubMed
2. 1. EMBO J. 1986 Dec 20;5(13):3509-16 - PubMed
3. 1. Mol Cell Biol. 1987 May;7(5):1731-9 - PubMed
4. 1. J Biol Chem. 1987 Jul 25;262(21):9931-4 - PubMed
5. 1. Cell. 1987 Sep 11;50(6):819-20 - PubMed

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