Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR - PubMed (original) (raw)

. 1988 Sep 16;241(4872):1481-5.

doi: 10.1126/science.3262235.

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Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR

N Ahmad et al. Science. 1988.

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Abstract

In studies of the genetics of human immunodeficiency virus type 1 (HIV-1), the product of the nef gene, formerly known as F, 3'-orf, or B-ORF, was a negative regulator of HIV-1 replication. Proviruses with mutations in the nef gene replicated better than their standard counterparts during transient expression, and the mutant virus maintained its enhanced replication even after serial passages in T lymphocytes. The nef protein trans-suppressed, in a dose-dependent manner, the replication of wild-type and nef mutant proviruses and the expression of reporter genes linked to the HIV-1 long terminal repeat (LTR). The repression induced by the nef protein was mediated by inhibition of transcription from the HIV-1 LTR, which contains a far upstream cis element (previously recognized to be a negative regulatory element) between 340 and 156 nucleotides upstream of the RNA initiation site.

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