Stereospecific mobilization of intracellular Ca2+ by inositol 1,4,5-triphosphate. Comparison with inositol 1,4,5-trisphosphorothioate and inositol 1,3,4-trisphosphate - PubMed (original) (raw)
Stereospecific mobilization of intracellular Ca2+ by inositol 1,4,5-triphosphate. Comparison with inositol 1,4,5-trisphosphorothioate and inositol 1,3,4-trisphosphate
J Strupish et al. Biochem J. 1988.
Abstract
The stereo specificity of myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] to mobilize Ca2+ from an intracellular store has been examined in permeabilized rat pituitary-tumour GH3 and Swiss 3T3 cells. A comparison of D-Ins(1,4,5)P3 with the synthetic enantiomer L-Ins(1,4,5)P3 and the racemate DL-Ins(1,4,5)P3 clearly demonstrates the marked stereospecificity of the response. Whereas D-Ins(1,4,5)P3 released 30-50% of non-mitochondrially-bound Ca2+ with a EC50 (concentration producing 50% of maximal response) of 200 nM, the L isomer was both substantially less potent and efficacious. A high concentration of the L isomer (10 microM) did not significantly shift the dose-response curve for the D isomer in Swiss 3T3 cells, suggesting that the less active isomer is probably a very weak agonist. Other studies revealed, in contrast with previous work, that the other naturally occurring isomer, D-Ins(1,3,4)P3, was essentially inactive in releasing Ca+, whereas a novel 5-phosphatase-resistant analogue, DL-myo-inositol 1,4,5-trisphosphorothioate, was a relatively potent full agonist in GH3 cells. These data reveal, for the first time, the stereoselectivity of the intracellular receptor associated with Ca2+ release. They also provide evidence for the activity of the novel phosphorothioate analogue of Ins(1,4,5)P3, but suggest that D-Ins(1,3,4)P3 is not involved in cellular Ca2+ mobilization.
Similar articles
- Inositol 1,4,5-trisphosphorothioate, a stable analogue of inositol trisphosphate which mobilizes intracellular calcium.
Taylor CW, Berridge MJ, Cooke AM, Potter BV. Taylor CW, et al. Biochem J. 1989 May 1;259(3):645-50. doi: 10.1042/bj2590645. Biochem J. 1989. PMID: 2786414 Free PMC article. - Synthetic phosphorothioate-containing analogues of inositol 1,4,5-trisphosphate mobilize intracellular Ca2+ stores and interact differentially with inositol 1,4,5-trisphosphate 5-phosphatase and 3-kinase.
Safrany ST, Wojcikiewicz RJ, Strupish J, McBain J, Cooke AM, Potter BV, Nahorski SR. Safrany ST, et al. Mol Pharmacol. 1991 Jun;39(6):754-61. Mol Pharmacol. 1991. PMID: 1646949 - Myo-inositol 1,3,4,5-tetrakisphosphate can independently mobilise intracellular calcium, via the inositol 1,4,5-trisphosphate receptor: studies with myo-inositol 1,4,5-trisphosphate-3-phosphorothioate and myo-inositol hexakisphosphate.
Wilcox RA, Whitham EM, Liu C, Potter BV, Nahorski SR. Wilcox RA, et al. FEBS Lett. 1993 Dec 27;336(2):267-71. doi: 10.1016/0014-5793(93)80817-e. FEBS Lett. 1993. PMID: 8262243 - Inositol polyphosphates and intracellular calcium release.
Joseph SK, Williamson JR. Joseph SK, et al. Arch Biochem Biophys. 1989 Aug 15;273(1):1-15. doi: 10.1016/0003-9861(89)90156-2. Arch Biochem Biophys. 1989. PMID: 2667466 Review. - Inositol trisphosphate, calcium and muscle contraction.
Somlyo AP, Walker JW, Goldman YE, Trentham DR, Kobayashi S, Kitazawa T, Somlyo AV. Somlyo AP, et al. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26;320(1199):399-414. doi: 10.1098/rstb.1988.0084. Philos Trans R Soc Lond B Biol Sci. 1988. PMID: 2906146 Review.
Cited by
- Semiconducting Carbon Nanotube-Based Nanodevices for Monitoring the Effects of Chlorphenamine on the Activities of Intracellular Ca2+ Stores.
Pham Ba VA, Pham Van Bach N, Nguyen Luong T, Nguyen KV. Pham Ba VA, et al. J Anal Methods Chem. 2022 Mar 2;2022:9019262. doi: 10.1155/2022/9019262. eCollection 2022. J Anal Methods Chem. 2022. PMID: 35284149 Free PMC article. - Pharmacological modulation of intracellular Ca(2+) channels at the single-channel level.
Koulen P, Thrower EC. Koulen P, et al. Mol Neurobiol. 2001 Aug-Dec;24(1-3):65-86. doi: 10.1385/MN:24:1-3:065. Mol Neurobiol. 2001. PMID: 11831555 Review. - Stereoselectivity of Ins(1,3,4,5)P4 recognition sites: implications for the mechanism of the Ins(1,3,4,5)P4-induced Ca2+ mobilization.
Wilcox RA, Challiss RA, Baudin G, Vasella A, Potter BV, Nahorski SR. Wilcox RA, et al. Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):191-4. doi: 10.1042/bj2940191. Biochem J. 1993. PMID: 8363572 Free PMC article. - ATP and the binding of [3H]inositol 1,4,5-trisphosphate to its receptor.
Willcocks AL, Nahorski SR. Willcocks AL, et al. Biochem J. 1988 Nov 1;255(3):1061. doi: 10.1042/bj2551061. Biochem J. 1988. PMID: 2850794 Free PMC article. No abstract available. - A simple enzymic method to separate [3H]inositol 1,4,5- and 1,3,4-trisphosphate isomers in tissue extracts.
Kennedy ED, Batty IH, Chilvers ER, Nahorski SR. Kennedy ED, et al. Biochem J. 1989 May 15;260(1):283-6. doi: 10.1042/bj2600283. Biochem J. 1989. PMID: 2789038 Free PMC article.
References
- Am J Physiol. 1978 Aug;235(2):E97-102 - PubMed
- Biochem Biophys Res Commun. 1988 Jan 29;150(2):626-32 - PubMed
- Nature. 1984 Nov 22-28;312(5992):315-21 - PubMed
- Biochem J. 1985 Jul 15;229(2):505-11 - PubMed
- Biochem Biophys Res Commun. 1985 Dec 17;133(2):532-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous