Ultra-high-resolution SD-OCM imaging with a compact polarization-aligned 840 nm broadband combined-SLED source - PubMed (original) (raw)

. 2020 Jun 1;11(6):3395-3406.

doi: 10.1364/BOE.394229.

Marcus Duelk 2, Stefan Gloor 2, Jean Dahdah 2, Jose Ojeda 2, Caterina Sturtzel 3, Shiyu Deng 1, Abigail Joyce Deloria 1, Qian Li 1, Mengyang Liu 1, Martin Distel 3, Wolfgang Drexler 1, Rainer Leitgeb 1 4

Affiliations

Ultra-high-resolution SD-OCM imaging with a compact polarization-aligned 840 nm broadband combined-SLED source

Richard Haindl et al. Biomed Opt Express. 2020.

Abstract

We analyze the influence of intrinsic polarization alignment on image quality and axial resolution employing a broadband 840 nm light source with an optical bandwidth of 160 nm and an output power of 12 mW tailored for spectral-domain optical coherence microscopy (SD-OCM) applications. Three superluminescent diodes (SLEDs) are integrated into a 14-pin butterfly module using a free-space micro-optical bench architecture, maintaining a constant polarization state across the full spectral output. We demonstrate superior imaging performance in comparison to traditionally coupled-SLED broadband light sources in a teleost model organism in-vivo.

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

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Conflict of interest statement

Marcus Duelk (FEP), Stefan Gloor (FEP), Jean Dahdah (FEP), José Ojeda (FEP) are collaborators of EXALOS AG. EXALOS provided light sources for testing to the Medical University of Vienna. The authors declare no other conflicts of interest.

Figures

Fig. 1.

Fig. 1.

Ultra-high-resolution SD-OCM system. (a) System sketch. (b) 14-pin butterfly combi-SLED module (yellow ellipse) on OEM driver board EBD9200. (c) Open combi-SLED module with 3 SLEDs (D1, D2, D3); DM: Dichroic mirror; L1 to L3: Collimation lenses; F: Optical fiber; L: Focusing lens; PD: Monitoring photodiode; Green, yellow and magenta dotted arrows: Optical beam path for the individual diodes D1, D2 and D3 respectively; Red dotted arrow: Common optical beam path.

Fig. 2.

Fig. 2.

ASE spectrum and calculated coherence function. (a, b): Linear ASE spectrum of the EBD29 and EBS30. (c, d): calculated linear coherence function of the EBD29 and EBS30. The corresponding bandwidths and calculated axial resolutions can be found in Table 1.

Fig. 3.

Fig. 3.

Polarization dependent spectral intensity variations of the EBD29 and EBS30 SLEDs measured with a commercial spectrometer (AVS-USB2000, Avantes). (a, b) Spectral intensity for the EBD29 and EBS30. Polarization state 1: magenta; Polarization state 2: blue; Polarization state 3: green. (c, d) Ratio between polarization state 1 and 3 (magenta), 1 and 2 (blue), 2 and 3 (green) for the EBD29 and EBS30.

Fig. 4.

Fig. 4.

Input polarization dependent OCM coherence function. The coherence functions are acquired at 500 µm distance to the zero delay. (a, b) Coherence function of the EBD29 and EBS30. Magenta: input polarization 1; blue: input polarization 2.

Fig. 5.

Fig. 5.

Interference pattern and coherence function for the EBD29 and EBS30 light sources for different quarter-wave plate orientations. (a) Graphical illustration for horizontally polarized light (H) traversing a quarter-wave plate (λ/4, 45° fast-axis orientation) a second time after being reflected by a mirror. V: vertically polarized light; Circular arrow: chirality of circularly polarized light. (b) Color coding for various fast-axis orientations of the quarter-wave plate. (c, d) Interference pattern produced by the EBD29 and EBS30 light source for three quarter-wave plate orientations. (e, f) Coherence functions produced by the EBD29 and EBS30 light source for three quarter-wave plate orientations. The coherence functions are acquired at 500 µm distance to the zero delay.

Fig. 6.

Fig. 6.

Averaged (10x) intensity B-scans of a zebrafish larva acquired for two polarization states with two light sources. Fiber polarization controller position 1 corresponds to a horizontal linear and position 2 to a circular polarization state. The polarization states are measured for the EBD29 light source with a PAX5710IR1-T TXP polarimeter. (a, b) Polarization state 1 and 2 acquired with the EBD29 light source. (c, d) Polarization state 1 and 2 acquired with the EBS30 light source. Blue marks: regions of interest; Yellow arrows: artifacts caused by PSF side lobes; Magenta arrows: polarization dependent PSF side lobe amplitudes; Green arrows: same speckle in all images; Green star: highest visibility for polarization state dependent signal amplitudes; Magenta triangles: caudal vein (left) and dorsal aorta (right); YS: yolk sack; MT: myotomes (large muscle segments); NC: notochord; SC: spinal cord

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