Dendritic cells from rat lung are potent accessory cells - PubMed (original) (raw)
Dendritic cells from rat lung are potent accessory cells
C L Rochester et al. Am Rev Respir Dis. 1988 Jul.
Abstract
Accessory cells are required for the induction of lymphocyte proliferation in response to mitogens or antigens. Rat pulmonary cells were tested for the presence of accessory activity in lymphocyte proliferation induced by sodium periodate. Buffer-perfused lungs were excised, minced, and enzymatically digested. The resulting pulmonary cells (PC) were separated into high density (HD-PC, 32 to 57%) and low density (LD-PC, 9 to 32%) fractions in a discontinuous density gradient of bovine plasma albumin (BPA). Both macrophages and dendritic cells were observed in the LD-PC by light microscopy. HD-PC, LD-PC, adherent LD-PC, nonadherent LD-PC, and a purified preparation of pulmonary dendritic cells (DC-P) were tested for accessory activity in the presence of periodate-treated, lymph-node-derived lymphocytes as responders. Most of the accessory activity was found in the LD-PC. Increasing numbers of LD-PC stimulated proliferation of responder lymphocytes in a linear, dose-dependent manner; higher numbers had a suppressive effect. Nonadherent LD-PC containing dendritic cells also produced a dose-dependent increase in periodate-induced lymphocyte proliferation, whereas adherent LD-PC, morphologically identified as macrophages, were suppressive. Removal of phagocytic macrophages from nonadherent LD-PC resulted in an eightfold increase in both the percent of DC-P present and the amount of accessory activity in the LD-PC. We conclude that pulmonary dendritic cells are potent accessory cells for periodate-induced lymphocyte proliferation.
Similar articles
- Accessory cell dependent T lymphocyte proliferation: potent activity of dendritic cells.
Goodell EM, Stoltenborg JK, Bowers WE. Goodell EM, et al. Immunobiology. 1987 Jan;174(1):30-42. doi: 10.1016/S0171-2985(87)80082-7. Immunobiology. 1987. PMID: 3494665 - The effect of silica treatment on accessory cell-dependent rat T lymphocyte proliferation.
Bowers WE, Ruhoff MS, Goodell EM, Stoltenborg JK. Bowers WE, et al. Immunobiology. 1988 Feb;176(3):179-94. doi: 10.1016/S0171-2985(88)80052-4. Immunobiology. 1988. PMID: 2834290 - T cell activation by antigen-presenting cells from lung tissue digests: suppression by endogenous macrophages.
Holt PG, Degebrodt A, O'Leary C, Krska K, Plozza T. Holt PG, et al. Clin Exp Immunol. 1985 Dec;62(3):586-93. Clin Exp Immunol. 1985. PMID: 3878750 Free PMC article. - Pulmonary immune cells in health and disease: dendritic cells and Langerhans' cells.
Hance AJ. Hance AJ. Eur Respir J. 1993 Sep;6(8):1213-20. Eur Respir J. 1993. PMID: 8224138 Review. - Granulocyte-macrophage colony-stimulating factor overrides the immunosuppressive function of corticosteroids on rat pulmonary dendritic cells.
Lim TK, Chen GH, McDonald RA, Toews GB. Lim TK, et al. Stem Cells. 1996 May;14(3):292-9. doi: 10.1002/stem.140292. Stem Cells. 1996. PMID: 8724695 Review.
Cited by
- High stimulatory activity of dendritic cells from diabetes-prone BioBreeding/Worcester rats exposed to macrophage-derived factors.
Tafuri A, Bowers WE, Handler ES, Appel M, Lew R, Greiner D, Mordes JP, Rossini AA. Tafuri A, et al. J Clin Invest. 1993 May;91(5):2040-8. doi: 10.1172/JCI116426. J Clin Invest. 1993. PMID: 8486773 Free PMC article. - Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages.
Holt PG, Oliver J, Bilyk N, McMenamin C, McMenamin PG, Kraal G, Thepen T. Holt PG, et al. J Exp Med. 1993 Feb 1;177(2):397-407. doi: 10.1084/jem.177.2.397. J Exp Med. 1993. PMID: 8426110 Free PMC article. - Induction of MHC class II antigens on rat bronchial epithelial cells by interferon-gamma and its effect on antigen presentation.
Suda T, Sato A, Sugiura W, Chida K. Suda T, et al. Lung. 1995;173(2):127-37. doi: 10.1007/BF02981472. Lung. 1995. PMID: 7715254 - The antigen-presenting activities of Ia+ dendritic cells shift dynamically from lung to lymph node after an airway challenge with soluble antigen.
Xia W, Pinto CE, Kradin RL. Xia W, et al. J Exp Med. 1995 Apr 1;181(4):1275-83. doi: 10.1084/jem.181.4.1275. J Exp Med. 1995. PMID: 7699319 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous