Breeding for three-line japonica hybrid rice combinations with high resistant starch content using molecular marker-assisted selection - PubMed (original) (raw)

Breeding for three-line japonica hybrid rice combinations with high resistant starch content using molecular marker-assisted selection

Ruifang Yang et al. Breed Sci. 2020 Jun.

Abstract

Resistant starch (RS) is beneficial for human health, and especially for diabetics. Considering the high cost and low productivity of the Jiangtangdao 1 rice variety with high RS content, breeding high RS rice varieties exhibiting high productivity is essential. A molecular marker-assisted selection strategy was applied to increase RS content in a three-line hybrid rice variety. The functional rice variety Jiangtangdao 1, which contains sbe3-rs (on chr2) that controls the RS content, was used as the high RS content donor parent. Subsequently, male sterile maintainer and restorer lines containing homozygous sbe3-rs were bred using molecular marker-assisted selection combined with traditional breeding methods. The male sterile line was crossed with the restorer lines to identify the optimal hybrid combination with a high RS content. We obtained four combinations for which the yields were >50% higher than those of the control Jiangtangdao 1. In addition, there was no significant difference in the RS content between the combinations and Jiangtangdao 1. The hybrid rice plants with high RS content exhibited favorable agronomic traits and therefore have broad prospects for commercial application.

Keywords: Jiangtangdao 1; hybrid rice; molecular marker-assisted selection; resistant starch; sbe3-rs.

Copyright © 2020 by JAPANESE SOCIETY OF BREEDING.

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Figures

Fig. 1.

Fig. 1.

Process of selecting maintainer and restorer lines with high RS content via the introgression of sbe3-rs (donor parent: Jiangtangdao 1) into maintainer line 49B and restorer line R1 using MAS approaches. MAS: marker assistance selection; RS: resistant starch.

Fig. 2.

Fig. 2.

Marker selection for sbe3-rs in the BC7F1 maintainer population using CAPS marker. Because the point mutation resulted in the loss of the restriction enzyme site of _Spe_I, the BC7F1 plants carrying sbe3-rs had three bands. M: maker DL2000, 1–12: 12 plants of the BC7F1 populations. 2, 4–8 are heterozygote genotypes. CAPS: cleaved amplified polymorphic sequence.

Fig. 3.

Fig. 3.

Scheme for selecting MS line carrying homozygous sbe3-rs (A) and pollen fertility analysis by potassium iodide staining (B: full pollen fertility control; C: RS49A). MS: male sterile. The full pollens of RS49A exhibiting sterility with starch grain filling and light color aborted.

Fig. 4.

Fig. 4.

Verification of the lines developed with homozygous sbe3-rs using CAPS marker. M: marker DL2000; L: low RS plant control; H: sbe3-rs homozygous genotype; 1: RS49A; 2: RS49B; 3–6: RSR307, RSR308, RSR309, and RSR286.

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