Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae - PubMed (original) (raw)
Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae
A R Buchman et al. Mol Cell Biol. 1988 Jan.
Abstract
Two DNA-binding factors from Saccharomyces cerevisiae have been characterized, GRFI (general regulatory factor I) and ABFI (ARS-binding factor I), that recognize specific sequences within diverse genetic elements. GRFI bound to sequences at the negative regulatory elements (silencers) of the silent mating type loci HML E and HMR E and to the upstream activating sequence (UAS) required for transcription of the MAT alpha genes. A putative conserved UAS located at genes involved in translation (RPG box) was also recognized by GRFI. In addition, GRFI bound with high affinity to sequences with the (C1-3A)-repeat region at yeast telomeres. Binding sites for GRFI with the highest affinity appeared to be of the form 5'-(A/G)(A/C)ACCCANNCA(T/C)(T/C)-3', where N is any nucleotide. ABFI-binding sites were located next to autonomously replicating sequences (ARSs) at controlling elements of the silent mating type loci HMR E, HMR I, and HML I and were associated with ARS1, ARS2, and the 2 micron plasmid ARS. Two tandem ABFI binding sites were found between the HIS3 and DED1 genes, several kilobase pairs from any ARS, indicating that ABFI-binding sites are not restricted to ARSs. The sequences recognized by ABFI showed partial dyad-symmetry and appeared to be variations of the consensus 5'-TATCATTNNNNACGA-3'. GRFI and ABFI were both abundant DNA-binding factors and did not appear to be encoded by the SIR genes, whose products are required for repression of the silent mating type loci. Together, these results indicate that both GRFI and ABFI play multiple roles within the cell.
Similar articles
- Roles of two DNA-binding factors in replication, segregation and transcriptional repression mediated by a yeast silencer.
Kimmerly W, Buchman A, Kornberg R, Rine J. Kimmerly W, et al. EMBO J. 1988 Jul;7(7):2241-53. doi: 10.1002/j.1460-2075.1988.tb03064.x. EMBO J. 1988. PMID: 3046937 Free PMC article. - A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.
Buchman AR, Kornberg RD. Buchman AR, et al. Mol Cell Biol. 1990 Mar;10(3):887-97. doi: 10.1128/mcb.10.3.887-897.1990. Mol Cell Biol. 1990. PMID: 2406570 Free PMC article. - Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.
Buchman AR, Lue NF, Kornberg RD. Buchman AR, et al. Mol Cell Biol. 1988 Dec;8(12):5086-99. doi: 10.1128/mcb.8.12.5086-5099.1988. Mol Cell Biol. 1988. PMID: 3072472 Free PMC article. - Silencers, silencing, and heritable transcriptional states.
Laurenson P, Rine J. Laurenson P, et al. Microbiol Rev. 1992 Dec;56(4):543-60. doi: 10.1128/mr.56.4.543-560.1992. Microbiol Rev. 1992. PMID: 1480108 Free PMC article. Review. - The dual role of autonomously replicating sequences as origins of replication and as silencers.
Rehman MA, Yankulov K. Rehman MA, et al. Curr Genet. 2009 Aug;55(4):357-63. doi: 10.1007/s00294-009-0265-7. Epub 2009 Jul 26. Curr Genet. 2009. PMID: 19633981 Review.
Cited by
- Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation in Saccharomyces cerevisiae.
Bondra ER, Rine J. Bondra ER, et al. Proc Natl Acad Sci U S A. 2023 Oct 3;120(40):e2304343120. doi: 10.1073/pnas.2304343120. Epub 2023 Sep 28. Proc Natl Acad Sci U S A. 2023. PMID: 37769255 Free PMC article. - Context dependent function of the transcriptional regulator Rap1 in gene silencing and activation in Saccharomyces cerevisiae.
Bondra ER, Rine J. Bondra ER, et al. bioRxiv [Preprint]. 2023 May 11:2023.05.08.539937. doi: 10.1101/2023.05.08.539937. bioRxiv. 2023. PMID: 37214837 Free PMC article. Updated. Preprint. - SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid.
Sosa Ponce ML, Remedios MH, Moradi-Fard S, Cobb JA, Zaremberg V. Sosa Ponce ML, et al. J Cell Biol. 2023 Jul 3;222(7):e202206061. doi: 10.1083/jcb.202206061. Epub 2023 Apr 12. J Cell Biol. 2023. PMID: 37042812 Free PMC article. - Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans.
Wang WH, Lai TX, Wu YC, Chen ZT, Tseng KY, Lan CY. Wang WH, et al. Microbiol Spectr. 2022 Dec 21;10(6):e0328522. doi: 10.1128/spectrum.03285-22. Epub 2022 Nov 23. Microbiol Spectr. 2022. PMID: 36416583 Free PMC article. - The DNA replication protein Orc1 from the yeast Torulaspora delbrueckii is required for heterochromatin formation but not as a silencer-binding protein.
Maria H, Rusche LN. Maria H, et al. Genetics. 2022 Aug 30;222(1):iyac110. doi: 10.1093/genetics/iyac110. Genetics. 2022. PMID: 35894940 Free PMC article.
References
- Cell. 1981 Nov;27(1 Pt 2):15-23 - PubMed
- Mol Cell Biol. 1984 Aug;4(8):1440-8 - PubMed
- Mol Cell Biol. 1986 Oct;6(10):3513-22 - PubMed
- Nature. 1984 Jul 12-18;310(5973):154-7 - PubMed
- J Mol Biol. 1984 Oct 5;178(4):815-34 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases