Comparison of two direct-count techniques for enumerating aquatic bacteria - PubMed (original) (raw)
Comparative Study
Comparison of two direct-count techniques for enumerating aquatic bacteria
W B Bowden. Appl Environ Microbiol. 1977 May.
Abstract
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference between the two techniques when Nuclepore filters were used (0.5 less than P less than 0.9). The average cell volume from this study area was 0.047 micron3. The estimated number of bacteria ranged from 10(6) to 10(7) bacteria per ml, representing from 4 to 40 mg of C per m3.
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References
- Appl Environ Microbiol. 1977 Apr;33(4):940-6 - PubMed
- Appl Environ Microbiol. 1977 May;33(5):1225-8 - PubMed
- Appl Microbiol. 1974 Oct;28(4):708-12 - PubMed
- Science. 1964 Jan 17;143(3603):249-50 - PubMed
- Appl Microbiol. 1968 Jun;16(6):932-4 - PubMed
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