An alternative approach to somatic cell gene therapy - PubMed (original) (raw)
An alternative approach to somatic cell gene therapy
D St Louis et al. Proc Natl Acad Sci U S A. 1988 May.
Abstract
Mouse primary skin fibroblasts were infected with a recombinant retrovirus containing human factor IX cDNA. Bulk infected cells capable of synthesizing and secreting biologically active human factor IX protein were embedded in collagen, and the implant was grafted under the epidermis. Sera from the transplanted mice contain human factor IX protein for at least 10-12 days. Loss of immunoreactive human factor IX protein in the mouse serum is not due to graft rejection. Instead, the mouse serum contains anti-human factor IX antibodies, which react with the protein. We suggest that retroviral-infected primary skin fibroblasts offer an alternative approach to somatic cell gene therapy.
Similar articles
- Long-term expression of human factor IX cDNA in rabbits.
Zhou JM, Qiu XF, Lu DR, Lu JY, Xue JL. Zhou JM, et al. Sci China B. 1993 Nov;36(11):1333-41. Sci China B. 1993. PMID: 8142022 - Expression of human factor IX cDNA in mice by implants of genetically modified skin fibroblasts from a hemophilia B patient.
Zhou JM, Dai YF, Qiu XF, Hou GY, Akira Y, Xue JL. Zhou JM, et al. Sci China B. 1993 Sep;36(9):1082-92. Sci China B. 1993. PMID: 8274202 - Retroviral-mediated gene transfer into mammalian cells.
Kohn DB, Kantoff PW, Eglitis MA, McLachlin JR, Moen RC, Karson E, Zwiebel JA, Nienhuis A, Karlsson S, O'Reilly R, et al. Kohn DB, et al. Blood Cells. 1987;13(1-2):285-98. Blood Cells. 1987. PMID: 3311223 Review. - [Construction of a retroviral vector expressing human factor IX].
Matsushita T, Emi N, Saito H. Matsushita T, et al. Rinsho Byori. 1992 Mar;Suppl 92:107-13. Rinsho Byori. 1992. PMID: 1583769 Review. Japanese. No abstract available.
Cited by
- Phenotypic correction of factor IX deficiency in skin fibroblasts of hemophilic dogs.
Axelrod JH, Read MS, Brinkhous KM, Verma IM. Axelrod JH, et al. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5173-7. doi: 10.1073/pnas.87.13.5173. Proc Natl Acad Sci U S A. 1990. PMID: 2367529 Free PMC article. - Sustained delivery of erythropoietin in mice by genetically modified skin fibroblasts.
Naffakh N, Henri A, Villeval JL, Rouyer-Fessard P, Moullier P, Blumenfeld N, Danos O, Vainchenker W, Heard JM, Beuzard Y. Naffakh N, et al. Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3194-8. doi: 10.1073/pnas.92.8.3194. Proc Natl Acad Sci U S A. 1995. PMID: 7724539 Free PMC article. - Expression of human factor IX in rat capillary endothelial cells: toward somatic gene therapy for hemophilia B.
Yao SN, Wilson JM, Nabel EG, Kurachi S, Hachiya HL, Kurachi K. Yao SN, et al. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8101-5. doi: 10.1073/pnas.88.18.8101. Proc Natl Acad Sci U S A. 1991. PMID: 1896457 Free PMC article. - Direct injection of a recombinant retroviral vector induces human immunodeficiency virus-specific immune responses in mice and nonhuman primates.
Irwin MJ, Laube LS, Lee V, Austin M, Chada S, Anderson CG, Townsend K, Jolly DJ, Warner JF. Irwin MJ, et al. J Virol. 1994 Aug;68(8):5036-44. doi: 10.1128/JVI.68.8.5036-5044.1994. J Virol. 1994. PMID: 8035504 Free PMC article. - Long-term production and delivery of human growth hormone in vivo.
Heartlein MW, Roman VA, Jiang JL, Sellers JW, Zuliani AM, Treco DA, Selden RF. Heartlein MW, et al. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10967-71. doi: 10.1073/pnas.91.23.10967. Proc Natl Acad Sci U S A. 1994. PMID: 7971993 Free PMC article.
References
- J Cell Biol. 1972 Sep;54(3):626-37 - PubMed
- J Cell Biol. 1963 May;17:299-313 - PubMed
- Proc Natl Acad Sci U S A. 1979 Mar;76(3):1274-8 - PubMed
- Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
- CRC Crit Rev Biochem. 1980;8(2):191-223 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources