Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor - PubMed (original) (raw)
Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
V de Lorenzo et al. J Bacteriol. 1987 Jun.
Abstract
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained the character of inducibility under iron starvation. A 250-bp fragment carrying this sequence was examined for protection against DNase I by the Fur protein, the product of a gene (fur) required for negative control of several iron-regulated functions. The DNase I footprints, in the presence of various divalent heavy-metal ions added as corepressors, revealed two contiguous binding sites with different lengths and affinities for Fur. Increased concentrations of the protein appeared to elicit formation of repressor oligomers which bind to the upstream and downstream regions of the P1 promoter in a metal-dependent fashion, but with a presently undefined stoichiometry. The primary site for Fur binding spans 31 bp and contains two overlapping symmetry dyads which share the sequence 5'-TCATT-3'. It also contains extensive homology with a 19-bp consensus sequence for iron-regulated genes as deduced from comparison with the fhuA and fepA putative promoter sequences.
Similar articles
- Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30.
de Lorenzo V, Giovannini F, Herrero M, Neilands JB. de Lorenzo V, et al. J Mol Biol. 1988 Oct 20;203(4):875-84. doi: 10.1016/0022-2836(88)90113-1. J Mol Biol. 1988. PMID: 3062182 - Mechanism and regulation of synthesis of aerobactin in Escherichia coli K12 (pColV-K30).
Neilands JB. Neilands JB. Can J Microbiol. 1992 Jul;38(7):728-33. doi: 10.1139/m92-119. Can J Microbiol. 1992. PMID: 1393837 Review. - Molecular mechanism of regulation of siderophore-mediated iron assimilation.
Bagg A, Neilands JB. Bagg A, et al. Microbiol Rev. 1987 Dec;51(4):509-18. doi: 10.1128/mr.51.4.509-518.1987. Microbiol Rev. 1987. PMID: 2963952 Free PMC article. Review. No abstract available.
Cited by
- Structural Perspectives on Metal Dependent Roles of Ferric Uptake Regulator (Fur).
Kang SM, Kang HS, Chung WH, Kang KT, Kim DH. Kang SM, et al. Biomolecules. 2024 Aug 9;14(8):981. doi: 10.3390/biom14080981. Biomolecules. 2024. PMID: 39199369 Free PMC article. Review. - The transcriptional regulation of the horizontally acquired iron uptake system, yersiniabactin and its contribution to oxidative stress tolerance and pathogenicity of globally emerging salmonella strains.
Diamant I, Adani B, Sylman M, Rahav G, Gal-Mor O. Diamant I, et al. Gut Microbes. 2024 Jan-Dec;16(1):2369339. doi: 10.1080/19490976.2024.2369339. Epub 2024 Jul 4. Gut Microbes. 2024. PMID: 38962965 Free PMC article. - General Overview of Klebsiella pneumonia: Epidemiology and the Role of Siderophores in Its Pathogenicity.
Abbas R, Chakkour M, Zein El Dine H, Obaseki EF, Obeid ST, Jezzini A, Ghssein G, Ezzeddine Z. Abbas R, et al. Biology (Basel). 2024 Jan 27;13(2):78. doi: 10.3390/biology13020078. Biology (Basel). 2024. PMID: 38392297 Free PMC article. Review. - The C-terminal domain of the ferric uptake regulator (Fur) binds a [2Fe-2S] cluster to sense the intracellular free iron content in Escherichia coli.
Fontenot CR, Ding H. Fontenot CR, et al. Biometals. 2023 Dec;36(6):1285-1294. doi: 10.1007/s10534-023-00517-6. Epub 2023 Jun 21. Biometals. 2023. PMID: 37344741 - Ferric uptake regulator (Fur) binds a [2Fe-2S] cluster to regulate intracellular iron homeostasis in Escherichia coli.
Fontenot CR, Ding H. Fontenot CR, et al. J Biol Chem. 2023 Jun;299(6):104748. doi: 10.1016/j.jbc.2023.104748. Epub 2023 Apr 24. J Biol Chem. 2023. PMID: 37100285 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1979 Oct;76(10):5061-5 - PubMed
- Mol Gen Genet. 1981;182(2):288-92 - PubMed
- Mol Gen Genet. 1983;191(2):301-6 - PubMed
- Annu Rev Nutr. 1981;1:27-46 - PubMed
- Annu Rev Biochem. 1984;53:293-321 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases