Two-color immunofluorescence using a fluorescence-activated cell sorter - PubMed (original) (raw)
Two-color immunofluorescence using a fluorescence-activated cell sorter
M R Loken et al. J Histochem Cytochem. 1977 Jul.
Abstract
A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.
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