A normal mitochondrial protein is selectively synthesized and accumulated during heat shock in Tetrahymena thermophila - PubMed (original) (raw)
A normal mitochondrial protein is selectively synthesized and accumulated during heat shock in Tetrahymena thermophila
T W McMullin et al. Mol Cell Biol. 1987 Dec.
Abstract
We have identified and purified a 58-kilodalton protein of Tetrahymena thermophila whose synthesis during heat shock parallels that of the major heat shock proteins. This protein, hsp58, was found in both non-heat-shocked as well as heat-shocked cells; however, its concentration in the cell increased approximately two- to threefold during heat shock. The majority of hsp58 in both non-heat-shocked and heat-shocked cells was found by both cell fractionation studies and immunocytochemical techniques to be mitochondrially associated. During heat shock, the additional hsp58 was found to selectively accumulate in mitochondria. Nondenatured hsp58 released from mitochondria of non-heat-shocked or heat-shocked cells sedimented in sucrose gradients as a 20S to 25S complex. We suggest that this protein may play a role in mitochondria analogous to the role the major heat shock proteins play in the nucleus and cytosol.
Similar articles
- A heat shock-induced, polymerase III-transcribed RNA selectively associates with polysomal ribosomes in Tetrahymena thermophila.
Kraus KW, Good PJ, Hallberg RL. Kraus KW, et al. Proc Natl Acad Sci U S A. 1987 Jan;84(2):383-7. doi: 10.1073/pnas.84.2.383. Proc Natl Acad Sci U S A. 1987. PMID: 3467363 Free PMC article. - Products of mitochondrial protein synthesis in Tetrahymena.
Young PG, Hunter NP. Young PG, et al. Can J Biochem. 1979 Apr;57(4):314-20. doi: 10.1139/o79-040. Can J Biochem. 1979. PMID: 445221 - Mitochondrial and cytoplasmic protein syntheses are not required for heat shock acquisition of ethanol and thermotolerance in yeast.
Watson K, Dunlop G, Cavicchioli R. Watson K, et al. FEBS Lett. 1984 Jul 9;172(2):299-302. doi: 10.1016/0014-5793(84)81145-x. FEBS Lett. 1984. PMID: 6378658 - Destabilization of tubulin mRNA during heat shock in Tetrahymena pyriformis.
Cóias R, Galego L, Barahona I, Rodrigues-Pousada C. Cóias R, et al. Eur J Biochem. 1988 Aug 15;175(3):467-74. doi: 10.1111/j.1432-1033.1988.tb14218.x. Eur J Biochem. 1988. PMID: 3137027 - Absence of heat shock protein synthesis in isolated mitochondria and plastids from maize.
Nieto-Sotelo J, Ho TH. Nieto-Sotelo J, et al. J Biol Chem. 1987 Sep 5;262(25):12288-92. J Biol Chem. 1987. PMID: 3624259
Cited by
- Chaperonin studies: faith, luck, and a little help from our friends.
Horwich AL. Horwich AL. Mol Biol Cell. 2017 Nov 1;28(22):2915-2918. doi: 10.1091/mbc.E17-07-0479. Mol Biol Cell. 2017. PMID: 29084908 Free PMC article. - Identification and metabolic characterization of the Zea mays mitochondrial homolog of the Escherichia coli groEL protein.
Prasad TK, Hallberg RL. Prasad TK, et al. Plant Mol Biol. 1989 Jun;12(6):609-18. doi: 10.1007/BF00044152. Plant Mol Biol. 1989. PMID: 24271194 - Calcium-dependent mitochondrial extrusion in ciliated protozoa.
Bisharyan Y, Clark TG. Bisharyan Y, et al. Mitochondrion. 2011 Nov;11(6):909-18. doi: 10.1016/j.mito.2011.08.001. Epub 2011 Aug 11. Mitochondrion. 2011. PMID: 21856451 Free PMC article. - Mitochondria Increase Three-Fold and Mitochondrial Proteins and Lipid Change Dramatically in Postmeristematic Cells in Young Wheat Leaves Grown in Elevated CO2.
Robertson EJ, Williams M, Harwood JL, Lindsay JG, Leaver CJ, Leech RM. Robertson EJ, et al. Plant Physiol. 1995 Jun;108(2):469-474. doi: 10.1104/pp.108.2.469. Plant Physiol. 1995. PMID: 12228485 Free PMC article.
References
- Cell. 1984 Mar;36(3):655-62 - PubMed
- J Gen Microbiol. 1971 Feb;65(2):209-23 - PubMed
- J Cell Biol. 1969 Apr;41(1):145-53 - PubMed
- Cell. 1986 Sep 12;46(6):873-83 - PubMed
- Cell. 1984 Oct;38(3):841-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources