Evaluation of electron-transfer flavoprotein and alpha-lipoamide dehydrogenase redox states by two-channel fluorimetry and its application to the investigation of beta-oxidation - PubMed (original) (raw)
Evaluation of electron-transfer flavoprotein and alpha-lipoamide dehydrogenase redox states by two-channel fluorimetry and its application to the investigation of beta-oxidation
W S Kunz. Biochim Biophys Acta. 1988.
Abstract
A new method permitting the simultaneous evaluation of the redox states of alpha-lipoamide dehydrogenase and electron-transfer flavoprotein in intact rat liver mitochondria by two-channel fluorimetry is described. It is shown that correction for the partial overlap of emission spectra can readily be introduced after a calibration procedure is performed. This method was applied to the investigation into regulation of palmitoylcarnitine oxidation. It was found that in the presence of rotenone, malonate and a redox buffer for the mitochondrial NAD-system, the beta-oxidation flux was sensitive to variations in redox state of respiratory chain electron carriers at low states of NAD reduction. Therefore, the concept of beta-oxidation control caused solely by the NAD redox state can no longer be sustained.
Similar articles
- Spectral properties of fluorescent flavoproteins of isolated rat liver mitochondria.
Kunz WS. Kunz WS. FEBS Lett. 1986 Jan 20;195(1-2):92-6. doi: 10.1016/0014-5793(86)80137-5. FEBS Lett. 1986. PMID: 3753688 - Quantification of the content of fluorescent flavoproteins in mitochondria from liver, kidney cortex, skeletal muscle, and brain.
Kunz WS, Gellerich FN. Kunz WS, et al. Biochem Med Metab Biol. 1993 Aug;50(1):103-10. doi: 10.1006/bmmb.1993.1051. Biochem Med Metab Biol. 1993. PMID: 8373630 - Contribution of different enzymes to flavoprotein fluorescence of isolated rat liver mitochondria.
Kunz WS, Kunz W. Kunz WS, et al. Biochim Biophys Acta. 1985 Sep 6;841(3):237-46. doi: 10.1016/0304-4165(85)90064-9. Biochim Biophys Acta. 1985. PMID: 4027266 - One- and two-electron oxidation-reduction properties of lipoamide dehydrogenase.
Bando Y, Aki K. Bando Y, et al. J Nutr Sci Vitaminol (Tokyo). 1992;Spec No:453-6. doi: 10.3177/jnsv.38.special_453. J Nutr Sci Vitaminol (Tokyo). 1992. PMID: 1338450 Review. No abstract available. - Activation of molecular oxygen by flavins and flavoproteins.
Massey V. Massey V. J Biol Chem. 1994 Sep 9;269(36):22459-62. J Biol Chem. 1994. PMID: 8077188 Review. No abstract available.
Cited by
- Sex and SP-A2 Dependent NAD(H) Redox Alterations in Mouse Alveolar Macrophages in Response to Ozone Exposure: Potential Implications for COVID-19.
Xu HN, Lin Z, Gandhi CK, Amatya S, Wang Y, Li LZ, Floros J. Xu HN, et al. Antioxidants (Basel). 2020 Sep 25;9(10):915. doi: 10.3390/antiox9100915. Antioxidants (Basel). 2020. PMID: 32992843 Free PMC article. - Quantitative redox imaging biomarkers for studying tissue metabolic state and its heterogeneity.
Xu HN, Li LZ. Xu HN, et al. J Innov Opt Health Sci. 2014 Mar;7(2):1430002. doi: 10.1142/S179354581430002X. J Innov Opt Health Sci. 2014. PMID: 31827630 Free PMC article. - Optical Redox Imaging Detects the Effects of DEK Oncogene Knockdown on the Redox State of MDA-MB-231 Breast Cancer Cells.
Wen Y, Xu HN, Privette Vinnedge L, Feng M, Li LZ. Wen Y, et al. Mol Imaging Biol. 2019 Jun;21(3):410-416. doi: 10.1007/s11307-019-01321-w. Mol Imaging Biol. 2019. PMID: 30758703 Free PMC article. - Optical Redox Imaging of Lonidamine Treatment Response of Melanoma Cells and Xenografts.
Xu HN, Feng M, Nath K, Nelson D, Roman J, Zhao H, Lin Z, Glickson J, Li LZ. Xu HN, et al. Mol Imaging Biol. 2019 Jun;21(3):426-435. doi: 10.1007/s11307-018-1258-z. Mol Imaging Biol. 2019. PMID: 30151646 Free PMC article. - The promiscuous enzyme medium-chain 3-keto-acyl-CoA thiolase triggers a vicious cycle in fatty-acid beta-oxidation.
Martines AMF, van Eunen K, Reijngoud DJ, Bakker BM. Martines AMF, et al. PLoS Comput Biol. 2017 Apr 3;13(4):e1005461. doi: 10.1371/journal.pcbi.1005461. eCollection 2017 Apr. PLoS Comput Biol. 2017. PMID: 28369071 Free PMC article.