Two distinct subpopulations of endosomes involved in membrane recycling and transport to lysosomes - PubMed (original) (raw)
Two distinct subpopulations of endosomes involved in membrane recycling and transport to lysosomes
S L Schmid et al. Cell. 1988.
Abstract
Functionally distinct subpopulations of endosomes involved in targeting internalized material to specific intracellular destinations were resolved as two distinct peaks by free-flow electrophoresis. The less anodally shifted peak contained a population of early endosomes selectively labeled by brief exposures to endocytic tracers or by receptor-bound transferrin. Late endosomes, labeled only after longer periods of internalization, migrated more toward the anode. While both fluid phase and certain receptor-bound markers could be rapidly chased from early to late endosomes, transferrin remained in vesicles comigrating with early endosomes even after prolonged uptake. Thus early and late endosomes are kinetically related but functionally distinct: early endosomes serve as the major site of recycling of membrane and surface receptors and late endosomes are involved in delivery to lysosomes. The subpopulations each contain unique polypeptides not found on the plasma membrane. Thus, endosomes cannot be derived entirely from internalized cell surface components, and may have at least partly independent biosynthetic origins.
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Marsh M, Schmid S, Kern H, Harms E, Male P, Mellman I, Helenius A. Marsh M, et al. J Cell Biol. 1987 Apr;104(4):875-86. doi: 10.1083/jcb.104.4.875. J Cell Biol. 1987. PMID: 3031085 Free PMC article. - Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump.
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Schmid S, Fuchs R, Kielian M, Helenius A, Mellman I. Schmid S, et al. J Cell Biol. 1989 Apr;108(4):1291-300. doi: 10.1083/jcb.108.4.1291. J Cell Biol. 1989. PMID: 2925786 Free PMC article. - Biosynthetic transport of a major lysosomal membrane glycoprotein, lamp-1: convergence of biosynthetic and endocytic pathways occurs at three distinctive points.
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Stoorvogel W, Strous GJ, Geuze HJ, Oorschot V, Schwartz AL. Stoorvogel W, et al. Cell. 1991 May 3;65(3):417-27. doi: 10.1016/0092-8674(91)90459-c. Cell. 1991. PMID: 1850321
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