Localization of proteins controlling motility and chemotaxis in Escherichia coli - PubMed (original) (raw)

Localization of proteins controlling motility and chemotaxis in Escherichia coli

H G Ridgway et al. J Bacteriol. 1977 Nov.

Abstract

Flagellar proteins controlling motility and chemotaxis in Escherichia coli were selectively labeled in vivo with [35S]methionine. This distribution of these proteins in subcellular fractions was examined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and autoradiography. The motA, motB, cheM, and cheD gene products were found to be confined exclusively to the inner cytoplasmic membrane fraction, whereas the cheY, cheW, and cheA (66,000 daltons) polypeptides appeared only in the soluble cytoplasmic fraction. The cheB, cheX, cheZ, and cheA (76,000 daltons) proteins, however, were distributed in both the cytoplasm and the inner membrane fractions. The hag gene product (flagellin) was the only flagellar protein examined that copurified with the outer lipopolysaccharide membrane. Differences in the intracellular locations of the che and mot gene prodcuts presumably reflect the functional attributes of these components.

PubMed Disclaimer

Similar articles

• Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells.
  Matsumura P, Silverman M, Simon M. Matsumura P, et al. J Bacteriol. 1977 Dec;132(3):996-1002. doi: 10.1128/jb.132.3.996-1002.1977. J Bacteriol. 1977. PMID: 336614 Free PMC article.
• Identification of polypeptides necessary for chemotaxis in Escherichia coli.
  Silverman M, Simon M. Silverman M, et al. J Bacteriol. 1977 Jun;130(3):1317-25. doi: 10.1128/jb.130.3.1317-1325.1977. J Bacteriol. 1977. PMID: 324984 Free PMC article.
• Polar location of the chemoreceptor complex in the Escherichia coli cell.
  Maddock JR, Shapiro L. Maddock JR, et al. Science. 1993 Mar 19;259(5102):1717-23. doi: 10.1126/science.8456299. Science. 1993. PMID: 8456299
• Integral membrane proteins required for bacterial motility and chemotaxis.
  Boyd A, Mandel G, Simon MI. Boyd A, et al. Symp Soc Exp Biol. 1982;35:123-37. Symp Soc Exp Biol. 1982. PMID: 6764039 Review.
• Chemotaxis and flagellar genes of Chromobacterium violaceum.
  Pereira M, Parente JA, Bataus LA, Cardoso Dd, Soares RB, Soares CM. Pereira M, et al. Genet Mol Res. 2004 Mar 31;3(1):92-101. Genet Mol Res. 2004. PMID: 15100991 Review.

Cited by

• Genome-Wide Screening of Oxidizing Agent Resistance Genes in Escherichia coli.
  Chen H, Wilson J, Ercanbrack C, Smith H, Gan Q, Fan C. Chen H, et al. Antioxidants (Basel). 2021 May 27;10(6):861. doi: 10.3390/antiox10060861. Antioxidants (Basel). 2021. PMID: 34072091 Free PMC article.
• Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont.
  Renoz F, Champagne A, Degand H, Faber AM, Morsomme P, Foray V, Hance T. Renoz F, et al. PeerJ. 2017 May 9;5:e3291. doi: 10.7717/peerj.3291. eCollection 2017. PeerJ. 2017. PMID: 28503376 Free PMC article.
• Behavioral responses to chemical cues by bacteria.
  Bartlett DH, Matsumura P. Bartlett DH, et al. J Chem Ecol. 1986 May;12(5):1071-89. doi: 10.1007/BF01638997. J Chem Ecol. 1986. PMID: 24307048
• The multiple signaling systems regulating virulence in Pseudomonas aeruginosa.
  Jimenez PN, Koch G, Thompson JA, Xavier KB, Cool RH, Quax WJ. Jimenez PN, et al. Microbiol Mol Biol Rev. 2012 Mar;76(1):46-65. doi: 10.1128/MMBR.05007-11. Microbiol Mol Biol Rev. 2012. PMID: 22390972 Free PMC article. Review.
• Motility and chemotaxis in alkaliphilic Bacillus species.
  Fujinami S, Terahara N, Krulwich TA, Ito M. Fujinami S, et al. Future Microbiol. 2009 Nov;4(9):1137-49. doi: 10.2217/fmb.09.76. Future Microbiol. 2009. PMID: 19895217 Free PMC article. Review.

References

1. 1. J Bacteriol. 1977 May;130(2):877-87 - PubMed
2. 1. J Bacteriol. 1977 Jun;130(3):1317-25 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):533-7 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4387-91 - PubMed
5. 1. Nature. 1976 Dec 9;264(5586):577-80 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology