Replication program of active and inactive multigene families in mammalian cells - PubMed (original) (raw)

Comparative Study

Replication program of active and inactive multigene families in mammalian cells

K S Hatton et al. Mol Cell Biol. 1988 May.

Abstract

In a comprehensive study, the temporal replication of tissue-specific genes and flanking sequences was compared in nine cell lines exhibiting different tissue-specific functions. Some of the rules we have determined for the replication of these tissue specific genes include the following. (i) Actively transcribed genes usually replicate during the first quarter of the S phase. (ii) Some immunoglobulin genes replicate during the first half of S phase even when no transcriptional activity is detected but appear to replicate even earlier in cell lines where they are transcribed. (iii) Nontranscribed genes can replicate during any interval of S phase. (iv) Multigene families arranged in clusters of 250 kilobases or less define a temporal compartment comprising approximately one-quarter of S phase. While these rules, and others that are discussed, apply to the tissue-specific genes studied here, all tissue-specific genes may not follow this pattern. In addition, housekeeping genes did not follow some of these rules. These results provide the first molecular evidence that the coordinate timing of replication of contiguous sequences within a multigene family is a general property of the mammalian genome. The relationship between replication very early during S phase and the transcriptional activity within a chromosomal domain is discussed.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3846-50 - PubMed
    1. Cell. 1981 Mar;23(3):731-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1023-7 - PubMed
    1. Cell. 1981 May;24(2):283-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources